BERBERINE DERIVATIVES AS CATIONIC FLUORESCENT-PROBES FOR THE INVESTIGATION OF THE ENERGIZED STATE OF MITOCHONDRIA
BERBERINE DERIVATIVES AS CATIONIC FLUORESCENT-PROBES FOR THE INVESTIGATION OF THE ENERGIZED STATE OF MITOCHONDRIA
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DOI：
10.1016/0005-2728(83)90122-6
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
DADAK, V
中科院分区：
文献类型：
--
作者：
MIKES, V;DADAK, V
研究了大鼠肝脏和牛心脏线粒体与一系列不同烷基链长度的荧光阳离子小檗碱衍生物的相互作用。衍生物的疏水性增加伴随着更大的分配系数值，并与线粒体内膜更疏水的区域结合。小檗碱可以作为在线粒体膜外表面发生的过程的敏感指标；在线粒体的激活状态下，13-甲基小檗碱获得最大的荧光增加（15倍）。荧光的增加是由于荧光量子产率的增加，尽管在通电后也可以检测到束缚导数的量的小幅增加。荧光与膜电位的大小呈线性关系。在观察到荧光增强的同时，发现旋转迁移率显著降低。小檗碱可以根据膜电位的极性在细胞膜内移动；因此，在激发态下，在较低极性区域中浸泡得越深，荧光增加越大。更多的疏水衍生物抑制大鼠肝脏线粒体中nad连锁呼吸，但对琥珀酸氧化无影响。米的浓度。
The interaction of rat liver and bovine heart mitochondria with a series of fluorescent, cationic berberine derivatives varying in the length of alkyl chain was investigated. An increase in the hydrophobicity of the derivative was accompanied by a larger value of the partition coefficient and by binding to a more hydrophobic region of the inner mitochondrial membrane. Berberines could be used as sensitive indicators of processes which take place on the outer surface of the mitochondrial membrane; the greatest (15-fold) increase in fluorescence was obtained with 13-methylberberine in the energized state of mitochondria. The fluorescence increase was due to the increase in fluorescence quantum yield although a small increase in the amount of bound derivative could also be detected upon energization. The fluorescence was linearly dependent on the magnitude of the membrane potential. In parallel with an observed fluorescence enhancement a considerable decrease in rotational mobility was found. Berberines may move in the inner membrane according to the polarity of the membrane potential; consequently, deeper immersion in the less polar region in the energized state brings about a larger fluorescence increase. More hydrophobic derivatives inhibited NAD-linked respiration in rat liver mitochondria but exerted no effect on succinate oxidation up to 10 .mu.M concentration.