Microfluidic techniques for high throughput single cell analysis.
Microfluidic techniques for high throughput single cell analysis.
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DOI：
10.1016/j.copbio.2016.02.015
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发表时间：
2016-08
影响因子：
7.7
通讯作者：
Oakey J
中科院分区：
文献类型：
--
作者：
Reece A;Xia B;Jiang Z;Noren B;McBride R;Oakey J
微流控系统的微制造和日益灵敏的分子放大工具的开发使单细胞分析平台得以小型化。直到最近，这些平台的吞吐量才增加到可以在单细胞水平上筛选种群的水平。基于主动和被动操作的技术现在能够区分单细胞表型，用于分选、诊断或预测各种临床方案的应用。多相微流体的引入使单细胞能够分割成生化离散的皮升环境。这些技术的结合使一类单细胞分析平台在数据驱动的生物医学、基因组学和转录组学方面具有巨大的潜力。
The microfabrication of microfluidic control systems and the development of increasingly sensitive molecular amplificaiton tools has enabled the miniaturization of single cells analytical platforms. Only recently has the throughput of these platforms increased to a level at which populations can be screened at the single cell level. Techniques based upon both active and passive maniuplation are now capable of discriminating between single cell phenotypes for sorting, diagnostic or prognostic applications in a variety of clinical scenarios. The introduction of multiphase microfluidics enables the segmentation of single cells into biochemically discrete picoliter environments. The combination of these techniques are enabling a class of single cell analytical platforms witin great potential for data driven biomedicine, genomics and transcriptomics.