Specificity of oral immune priming in the red flour beetle Tribolium castaneum
Specificity of oral immune priming in the red flour beetle Tribolium castaneum
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DOI：
10.1098/rsbl.2017.0632
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发表时间：
2017-12-01
期刊：
影响因子：
3.3
通讯作者：
Kurtz, Joachim
中科院分区：
文献类型：
--
作者：
Futo, Momir;Sell, Marie P.;Kurtz, Joachim
免疫特异性是指宿主的免疫系统对各种病原体或抗原变异体的区分程度。由于抗体反应，脊椎动物的免疫记忆具有高度的特异性。另一方面，一些无脊椎动物表现出免疫启动，即在二次接触以前遇到的病原体后，提高了存活率。到目前为止，只有通过败血症感染途径或使用活的病原体进行引发时，才能证明启动的特异性。因此，我们对红粉甲虫的口服引发途径进行了特异性检测。在引发方面，我们使用了来自三种不同昆虫病原苏云金芽孢杆菌的无病原体上清液，这三种上清液表达了不同的Cry毒素变体，以对这种甲虫的毒性而闻名。随后接触受感染的孢子表明，口服引爆对两个自然产生的菌株是特异的，而第三个工程菌株不会产生任何引爆效应。我们的数据表明，用非感染性细菌剂进行口服免疫引发可以是特异的，但引发效果并不是所有细菌株都通用的。
Immune specificity is the degree to which a host's immune system discriminates among various pathogens or antigenic variants. Vertebrate immune memory is highly specific due to antibody responses. On the other hand, some invertebrates show immune priming, i.e. improved survival after secondary exposure to a previously encountered pathogen. Until now, specificity of priming has only been demonstrated via the septic infection route or when live pathogens were used for priming. Therefore, we tested for specificity in the oral priming route in the red flour beetle, Tribolium castaneum. For priming, we used pathogen-free supernatants derived from three different strains of the entomopathogen, Bacillus thuringiensis, which express different Cry toxin variants known for their toxicity against this beetle. Subsequent exposure to the infective spores showed that oral priming was specific for two naturally occurring strains, while a third engineered strain did not induce any priming effect. Our data demonstrate that oral immune priming with a non-infectious bacterial agent can be specific, but the priming effect is not universal across all bacterial strains.