High glucose levels impact visual response properties of retinal ganglion cells in C57 mice—An in vitro physiological study
High glucose levels impact visual response properties of retinal ganglion cells in C57 mice—An in vitro physiological study
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DOI：
10.1007/s11427-017-9106-6
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发表时间：
2017-11
期刊：
影响因子：
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通讯作者：
Yuan Zhou;C. Xiao;M. Pu
中科院分区：
文献类型：
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作者：
Yuan Zhou;C. Xiao;M. Pu
本研究探讨了高糖条件下视网膜神经节细胞（RGCs）的视觉反应特性。记录来自小鼠视网膜的RGCs的细胞外单单位反应。并将眼杯制备为记录室中的平坦安装件并灌流艾姆斯培养基。在HG条件下，ON组RGC的RF平均大小（34.1± 2.9，n =14）显著小于OFF组RGC的RF平均大小（49.3± 0.3，n =12）（P<0.0001）。在渗透压对照组（HM）中，ON和OFF RGC之间也观察到相同的减少模式（P<0.0001）。高、高亮度组ON RGCs的平均亮度阈值（LT）均显著升高（高亮度组：P<0.0001;高亮度组：P<0.0002）。在相同条件下，OFF RGCs表现出类似的反应模式（HG：P<0.01; HM：P<0.0002）。在HM处理下，ON细胞的平均对比度增益显著低于OFF细胞的平均对比度增益（P<0.015，非配对Student’s t检验）。HG处理后ON细胞的平均对比度增益显著高于OFF细胞（P<0.0001）。本研究结果表明，HG减少感受野中心的大小，抑制亮度阈值，并减弱对比度增益的RGCs。HG对ON和OFF RGCs的影响可能通过不同的机制介导。
This study investigated visual response properties of retinal ganglion cells (RGCs) under high glucose levels. Extracellular single-unit responses of RGCs from mouse retinas were recorded. And the eyecup was prepared as a flat mount in a recording chamber and superfused with Ames medium. The averaged RF size of the ON RGCs (34.1±2.9,n=14) was significantly smaller than the OFF RGCs under the HG (49.3±0.3,n=12) (P<0.0001) conditions. The same reduction pattern was also observed in the osmotic control group (HM) between ON and OFF RGCs (P<0.0001). The averaged luminance threshold (LT) of ON RGCs increased significantly under HG or HM (HG:P<0.0001; HM:P<0.0002). OFF RGCs exhibited a similar response pattern under the same conditions (HG:P<0.01; HM:P<0.0002). The averaged contrast gain of ON cells was significantly lower than that of OFF cells with the HM treatment (P<0.015, unpaired Student’s t test). The averaged contrast gain of ON cells was significantly higher than OFF cells with the HG treatment (P<0.0001). The present results suggest that HG reduced receptive field center size, suppressed luminance threshold, and attenuated contrast gain of RGCs. The impact of HG on ON and OFF RGCs may be mediated via different mechanisms.