Amphiphilic helical peptide enhances the uptake of single-walled carbon nanotubes by living cells
Amphiphilic helical peptide enhances the uptake of single-walled carbon nanotubes by living cells
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DOI：
10.3181/0612-rm-284
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发表时间：
2007-10-01
影响因子：
3.2
通讯作者：
Pantano, Paul
中科院分区：
文献类型：
--
作者：
Chin, Shook-Fong;Baughman, Ray H.;Pantano, Paul
碳纳米管（CNTs）在活细胞中的应用，如细胞内传感器和纳米载体的成功与否，将取决于细胞吸收了多少碳纳米管。在这里，我们报告了HeLa细胞对单壁碳纳米管的吸收增强，单壁碳纳米管被设计的称为nano-1的肽包被。原子力显微镜显示，分散体由单个和小束纳米-1碳纳米管组成，直径为0.7- 32nm，长度为100- 400nm。光谱表征表明，纳米-1以非共价方式分散碳纳米管，保持碳纳米管的光学性质。元素分析表明，我们的样品制备方案包括超声和离心有效地消除了与碳纳米管制造过程相关的金属杂质。我们进一步表明，纯化的碳纳米管分散体以时间和温度依赖的方式被HeLa细胞吸收，并且它们不影响HeLa细胞的生长速率，这表明在这些条件下，细胞内的碳纳米管是无毒的。最后，我们发现，与不含多肽的血清培养液相比，在纳米-1存在的情况下，细胞吸收的碳纳米管大约多6倍。用肽包覆碳纳米管可以增强吸收，这为提高需要碳纳米管在细胞内的应用性能提供了一种策略。
The success of many projected applications of carbon nanotubes (CNTs) to living cells, such as intracellular sensors and nanovectors, will depend on how many CNTs are taken up by cells. Here we report the enhanced uptake by HeLa cells of single-walled CNTs coated with a designed peptide termed nano-1. Atomic force microscopy showed that the dispersions were composed of individual and small bundles of nano-1 CNTs with 0.7- to 32-nm diameters and 100- to 400-nm lengths. Spectroscopic characterizations revealed that nano-1 disperses CNTs in a non-covalent fashion that preserves CNT optical properties. Elemental analyses indicated that our sample preparation protocol involving sonication and centrifugation effectively eliminated metal impurities associated with CNT manufacturing processes. We further showed that the purified CNT dispersions are taken up by HeLa cells in a time- and temperature-dependent fashion, and that they do not affect the HeLa cell growth rate, evidence that the CNTs inside cells are not toxic under these conditions. Finally, we discovered that similar to 6-fold more CNTs are taken up by cells in the presence of nano-1 compared with medium containing serum but no peptide. The fact that coating CNTs with a peptide enhances uptake offers a strategy for improving the performance of applications that require CNTs to be inside cells.