Development of ten microsatellite loci for Gentiana crassicaulis (Gentianaceae)
Development of ten microsatellite loci for Gentiana crassicaulis (Gentianaceae)
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DOI：
10.1007/s10592-007-9313-3
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Ge, Xue-Jun
中科院分区：
文献类型：
--
作者：
Li, Yong;Li, Lin-Feng;Ge, Xue-Jun
粗茎龙胆是我国著名的中草药之一。其根源在于过度采集，导致其急剧减少。为了制定适当的保护和管理策略，这是很重要的，它的遗传多样性和了解其种群结构。在这里，10个多态性微卫星标记已经开发。结合生物素捕获法富集AC/TG微卫星。每个基因座的多态性进行了评估，在30个人从6个群体。等位基因数为2 ~ 9个，期望杂合度为0.32 ~ 0.78。
Genetiana crassicaulis is one of famous Chinese medicinal plant. The over-collection for its root has caused its dramatic reduction. In order to devise adequate conservation and management strategies for this species, it is important to characterize its genetic diversity and understand its population structure. Here, 10 polymorphic microsatellite markers have been developed. AC/TG microsatellite was enriched by combining biotin capture method. Polymorphism of each locus was assessed in 30 individuals from six populations. The number of alleles ranged from 2 to 9 and the expected heterozygosity ranged from 0.32 to 0.78.