Synthesis, characterizations of silica-coated gold nanorods and its applications in electroanalysis of hemoglobin
Synthesis, characterizations of silica-coated gold nanorods and its applications in electroanalysis of hemoglobin
复制标题
二氧化硅包覆金纳米棒的合成、表征及其在血红蛋白电分析中的应用
DOI：
10.1016/j.elecom.2007.12.017
复制
发表时间：
2008-03-01
影响因子：
5.4
通讯作者：
Zhu, Jun-Jie
中科院分区：
文献类型：
--
作者：
Zhang, Jing-Jing;Liu, Yu-Ge;Zhu, Jun-Jie
采用种子介导法合成了金纳米棒(GNR)，然后用正硅酸乙酯(TEOS)进行聚合，在金纳米棒核心周围形成了一层二氧化硅。透射电子显微镜照片显示，包覆了二氧化硅的金纳米棒(GNRs@SiO_2)分散均匀，平均长径比为3.1，二氧化硅壳层厚度均匀。进一步将核/壳纳米复合材料作为固定化血红蛋白的有效载体，制备了一种新型生物传感器。固定化的Hb对其血红素Fe(III)到Fe(II)氧化还原对的电子转移有增强作用。该传感器对H_2O_2具有良好的生物电催化活性，线性范围为8.0×10~(-7)~6.1×10~(-5)M，检测下限为6.0×10~(-8)M。计算得到固定化血红蛋白的表观米氏常数为0.13 mm。(C)2008爱思唯尔B.V.保留所有权利。
Gold nanorods (GNRs) were synthesized by a seed-mediated growth approach followed by TEOS polymerization leading to the formation of silica layer surrounding the gold nanorod core. TEM images showed that the silica-coated gold nanorods (GNRs@SiO2) were dispersed with an average aspect ratio of 3.1 for the GNRs cores and a uniform thickness of the silica shell. The core/shell nanocomposites were further used as efficient supports for the immobilization of hemoglobin (Hb) to fabricate a novel biosensor. The immobilized Hb showed an enhanced electron transfer for its heme Fe(III) to Fe(II) redox couple. This biosensor showed an excellent bioelectrocatalytic activity towards H2O2 with a linear range from 8.0 x 10(-7) to 6.1 x 10(-5) M, and the detection limit was 6.0 x 10(-8) M at 3 sigma. The apparent Michaelis-Menten constant of the immobilized hemoglobin was calculated to be 0.13 mM. (C) 2008 Elsevier B.V. All rights reserved.