CALORIMETRIC INVESTIGATION OF SINGLE STRANDED BASE STACKING IN RIBO-OLIGONUCLEOTIDE-A7
CALORIMETRIC INVESTIGATION OF SINGLE STRANDED BASE STACKING IN RIBO-OLIGONUCLEOTIDE-A7
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DOI：
10.1016/0301-4622(77)87023-3
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发表时间：
1977-01-01
影响因子：
3.8
通讯作者：
STURTEVANT, JM
中科院分区：
文献类型：
--
作者：
BRESLAUER, KJ;STURTEVANT, JM
Differential scanning calorimetry was employed to determine the energy change associated with single stranded base stacking in the ribo-oligonucleotide A7. A total enthalpy change of 20.3 kcal (mole of heptamer)-1 was measured. This corresponds to 2.9 kcal (mole of adenine)-1 or 3.4 kcal (mole of A-A stack)-1 if one assumes that all 6 stacking interactions are energetically equivalent. These results represent the 1st direct determination of this important parameter for a ribo-oligonucleotide. The calorimetrically determined value reported here is considerably lower than any of the previously publised van''t Hoff enthalpies but is consistent with values that can be derived from other calorimetric data.
