Mesenchymal Stem Cell‐Laden Composite β Cell Porous Microgel for Diabetes Treatment
Mesenchymal Stem Cell‐Laden Composite β Cell Porous Microgel for Diabetes Treatment
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DOI：
10.1002/adfm.202211897
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发表时间：
2023-02
影响因子：
19
通讯作者：
Ji Sun;Jingbo Li;Zhikun Huan;S. Pandol;Dechen Liu;Luoran Shang;Ling Li
中科院分区：
文献类型：
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作者：
Ji Sun;Jingbo Li;Zhikun Huan;S. Pandol;Dechen Liu;Luoran Shang;Ling Li
1型糖尿病是一种慢性代谢性疾病，其特征在于胰腺β细胞的自身免疫损伤。作为胰岛素的连续外源性胰岛素给药的替代方案，β细胞移植显示出提供了控制糖尿病高血糖症的替代方法。然而，β细胞移植面临着低β细胞增殖和免疫损伤的重大挑战。因此，非常需要能够促进胰岛微环境可持续性的新方法。本研究开发了负载间充质干细胞（MSC）的复合β细胞多孔微凝胶（MG），以解决糖尿病治疗的可持续性。MG通过微流体液滴模板制备，微流体液滴模板用致孔剂包封MSC和β细胞以产生多孔结构。除了为营养输送提供合适的微环境外，多孔结构还促进β细胞生长和胰岛素分泌。MSC突出的抗凋亡和免疫调节作用进一步为β细胞存活和功能提供了有利的环境。在此基础上，证明了MG在降低糖尿病小鼠的高血糖症和实现持续血糖控制方面的治疗性能。总的来说，这些结果表明，新型MG具有治疗糖尿病的巨大潜力，为临床β细胞移植提供了有希望的平台。
Type 1 diabetes mellitus is a chronic metabolic condition characterized by the autoimmune damage of pancreatic β cells. As an alternative to continuous exogenous insulin administration of insulin, β cell transplantation is shown to provide an alternative approach for controlling hyperglycemia in diabetes. However, β cell transplantation faces significant challenges of low β cell proliferation and immunologic insults. Thus, novel approaches capable of promoting the islet microenvironment for sustainability is highly desired. This study develops mesenchymal stem cell (MSC)‐laden composite β cell porous microgels (MGs) to address sustainability for the treatment of diabetes. The MGs are prepared via microfluidic droplet templates encapsulating both MSCs and β cells with porogen for creating a porous structure. In addition to offering a suitable microenvironment for nutrient delivery, the porous structure promoted β cell growth and insulin secretion. The outstanding anti‐apoptotic and immunomodulatory roles of MSCs further provide a favorable environment for β cell survival and function. On this basis, the therapeutic performance of the MGs in reducing hyperglycemia and achieving sustained glycemic control in diabetic mice is demonstrated. Collectively, these results show that the novel MGs have great potential for the treatment of diabetes providing a promising platform for clinical β cell transplantation.