Physical stress by magnetic force accelerates differentiation of human osteoblasts
Physical stress by magnetic force accelerates differentiation of human osteoblasts
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DOI：
10.1016/j.bbrc.2003.09.156
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发表时间：
2003-11-07
影响因子：
3.1
通讯作者：
Kataoka, K
中科院分区：
文献类型：
--
作者：
Yuge, L;Okubo, A;Kataoka, K
我们研究了磁力对培养的人成骨细胞分化的影响。将磁性微粒（MP）导入人成骨细胞系的细胞质中，MP-MF组将细胞置于磁场（MF）中培养。采用三组对照：不含MP的细胞在MF外培养（C组），不含MP的细胞在MF中培养（MF组），以及含MP的细胞在MF外培养（MP组）。MP-MF组细胞体积增大，沿磁极轴向沿着伸长。碱性磷酸酶（Alcohol）活性的出现、骨结节的形成和钙沉积的加速取决于磁场的强度。其他三组需要更长的培养时间才能显示出这些变化。核心结合因子A1（Cbfa 1：成骨细胞分化的转录因子）和骨钙素（一种参与控制骨生成的骨基质蛋白）在MP-MF组中的表达早于或强于其他组。比较MP-MF组和C组细胞丝裂原活化蛋白激酶（MAPK）的磷酸化水平。MP-MF组p38（MAPK）（p38）磷酸化水平明显升高，而总p38、MAPK/ERK 1/2和SAPK/JNK的总磷酸化水平和磷酸化水平在两组间无明显变化。C组在培养液中加入p38抑制剂SB 203580后，Alcohol活性、骨结节形成和钙沉积均被完全抑制。另一方面，MAPK/ERK 1/2抑制剂U-0126仅部分抑制它们。基于这些结果，可以得出结论：（1）成骨细胞分化是由磁力加速，（2）这种加速主要归因于p38磷酸化的激活，和（3）由磁场诱导的刺激提供了一种新的方法，成骨细胞分化。(C)2003年爱思唯尔公司All rights reserved.
We examined the effect of magnetic force on differentiation of cultured human osteoblasts. Magnetic microparticles (MPs) were introduced into the cytoplasm of a human osteoblast cell line and the cells were cultured in a magnetic field (MF) in group MP-MF. Three groups of controls were used: cells without MPs were cultured out of MF (group C), cells without MPs were cultured in MF (group MF), and cells with MPs were cultured out of MF (group MP). The cells in group MP-MF became larger and were elongated along the axis of the magnetic poles. Appearance of alkaline phosphatase (AlPase) activity, formation of bone nodules, and calcium deposition were accelerated depending on the intensity of the magnetic field. It takes longer culture in the other three groups to exhibit these changes. Core-binding factor A1 (Cbfa1: transcription factor for osteoblast differentiation) and osteocalcin (a bone-matrix protein involved in controlling osteogenesis) were expressed earlier or stronger in group MP-MF than the other groups. Then we compared phosphorylation of mitogen-activated protein kinase (MAPK) between group MP-MF and group C. Phosphorylation of p38(MAPK) (p38) was increased in group MP-MF, while total p38 as well as total and phosphorylated forms of MAPK/ERK 1/2 and SAPK/JNK were not changed between the two groups. When a p38 inhibitor, SB 203580, was added to the culture medium in group C, AlPase activity, formation of bone nodules, and calcium deposits were completely inhibited. On the other hand, they were inhibited only partially by a MAPK/ERK 1/2 inhibitor, U-0126. Based on these results, it is concluded that (1) osteoblast differentiation is accelerated by a magnetic force, (2) this acceleration is mainly attributed to the activation of p38 phosphorylation, and (3) the stimulus induced by a magnetic field offers a new approach to osteoblast differentiation. (C) 2003 Elsevier Inc. All rights reserved.