Gas phase electrochemical analysis of amino acids and their fragments
Gas phase electrochemical analysis of amino acids and their fragments
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DOI：
10.1038/s42004-018-0046-7
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发表时间：
2018-08
影响因子：
5.9
通讯作者：
M. Calleja;A. Elahi;D. Caruana
中科院分区：
文献类型：
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作者：
M. Calleja;A. Elahi;D. Caruana
所有的化学反应都涉及到分子内部或分子之间的电子重排。这些变化最好通过电化学等方法来研究，但这些方法主要用于液体和固体，而不是气体。这种排除限制了我们对等离子体系统中电子转移过程的理解，而等离子体系统具有很高的科学、工业和环境重要性。在这里，我们描述了在气相中包含的火焰等离子体中的小有机分子的电化学测量，通过使用循环伏安法探测产生的化学碎片的氧化还原活性。通过固/气界面的特定电子转移反应，记录了8种氨基酸及其片段的独特电流-电压谱。我们使用碎片的稳定类似物和原位质谱识别和分配碎片的电流-电压谱中的法拉第峰。我们表明，这种方法提供了有机分子的明确鉴定，具有与质谱相媲美的物种形成的灵敏度和能力。
All chemical reactions involve electron rearrangement within or between molecules. The changes are best studied by methods such as electrochemistry, but these have been developed mainly for liquids and solids rather than gases. This exclusion limits our understanding of electron transfer processes that are central in plasma systems, which are of high scientific, industrial, and environmental importance. Here we describe electrochemical measurements in the gas phase of small organic molecules contained in flame plasma, by probing the redox activity of the resulting chemical fragments using cyclic voltammetry. Unique current-voltage spectra are recorded for eight amino acids and their fragments, through specific electron transfer reactions at the solid/gas interface. We identify and assign Faradaic peaks in the current-voltage spectra to the fragments using stable analogues of the fragments and in situ mass spectroscopy. We show that this approach provides unambiguous identification of organic based molecules, with a sensitivity and power of speciation to rival mass spectrometry.