Interleukin-15: a novel anabolic cytokine for skeletal muscle.
Interleukin-15: a novel anabolic cytokine for skeletal muscle.
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DOI：
10.1210/endo.136.8.7628408
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发表时间：
1995-08
期刊：
影响因子：
4.8
通讯作者：
L S Quinn;K. Haugk;K. Grabstein
中科院分区：
文献类型：
--
作者：
L S Quinn;K. Haugk;K. Grabstein
白细胞介素15(IL-15)是新近发现的一种生长因子，在骨骼肌中高表达。为探讨IL-15在骨骼肌发生中的作用，采用小鼠C2骨骼肌成肌细胞系和原代胎牛骨骼肌成肌细胞系，研究了IL-15对成肌细胞增殖和肌球蛋白重链(MHC)表达的影响。IL-15对[~3H]胸腺嘧啶核苷掺入和抗MHC免疫细胞化学染色检测的成肌细胞分化率均无影响。然而，Western印迹分析显示，浓度为10或100 ng/ml的IL-15使C2成肌细胞培养的MHC积累量增加5倍，在原代牛成肌细胞培养的MHC积累量增加2.5倍。此外，在IL-15存在下形成的C2肌管明显大于对照组。这些发现表明，IL-15可以刺激分化的肌细胞和肌纤维积累更多的收缩蛋白。用有丝分裂抑制剂aphidiclin处理融合良好的原代牛成肌细胞，然后加入IL-15和/或合成生长因子胰岛素样生长因子-I(IGF-I)，用Western blotting分析MHC的积累。IL-15浓度为10 ng/ml时，MHC积累量增加了一倍，与IGF-I浓度为10或100 ng/ml时的效果相同。IL-15和IGF-I联合使用可使MHC积累量增加近5倍，表明这两种因子对肌纤维的作用是相加的。这些发现表明，IL-15影响与骨骼肌纤维肥大相关的参数，并提示IL-15可能是一种增加骨骼肌质量的新型合成代谢剂。
Interleukin-15 (IL-15) is a recently discovered growth factor which is highly expressed in skeletal muscle. In order to determine a functional role for IL-15 in skeletal myogenesis, the effects of IL-15 on myoblast proliferation and muscle-specific myosin heavy chain (MHC) expression were analyzed using the mouse C2 skeletal myogenic cell line and primary fetal bovine skeletal myogenic cultures. IL-15 had no effect on [3H]thymidine incorporation, nor on the rate of myoblast differentiation, assessed by anti-MHC immunocytochemical staining, in either type of culture. However, Western blot analyses revealed that IL-15 used at concentrations of 10 or 100 ng/ml increased MHC accumulation five-fold in C2 myoblast cultures and 2.5-fold in primary bovine myogenic cultures. Moreover, C2 myotubes formed in the presence of IL-15 appeared larger than controls. These findings indicate IL-15 can stimulate differentiated myocytes and muscle fibers to accumulate increased amounts of contractile proteins. Well-fused primary bovine myogenic cultures treated with the mitotic inhibitor aphidicolin, then administered IL-15 and/or the anabolic growth factor insulin-like growth factor-I (IGF-I), were analyzed for MHC accumulation using Western blots. IL-15 used at 10 ng/ml doubled MHC accumulation and was as effective as IGF-I used at 10 or 100 ng/ml. IL-15 and IGF-I used together increased MHC accumulation close to five-fold, indicating these two factors can act additively on muscle fibers. These findings indicate IL-15 affects parameters associated with skeletal muscle fiber hypertrophy, and suggest that IL-15 may be a novel anabolic agent to increase skeletal muscle mass.