A novel method to control the balance of skin microflora -: Part 1.: Attack on biofilm of Staphylococcus aureus without antibiotics
A novel method to control the balance of skin microflora -: Part 1.: Attack on biofilm of Staphylococcus aureus without antibiotics
复制标题
DOI：
10.1016/j.jdermsci.2005.01.006
复制
发表时间：
2005-06-01
影响因子：
4.6
通讯作者：
Zenro, I
中科院分区：
文献类型：
--
作者：
Masako, K;Hideyuki, I;Zenro, I
背景：金黄色葡萄球菌（Staphylococcus aureus，SA）通常存在于特应性干燥皮肤中，而不仅仅存在于特应性皮炎严重影响的区域。SA释放各种毒素和酶，损伤皮肤，并形成生物膜纤维蛋白纤维和糖萼;生物膜是重要的SA粘附到皮肤和抗微生物剂。即使是非常有效的保湿剂也不能完美地作用于特应性干燥皮肤。表皮葡萄球菌（Staphylococcus epidermidis，SE）是健康人体皮肤微生物群落的主要组成部分，对致病菌的生长具有保护作用。目的：由于抗微生物药物治疗可能导致SA的再生长，SA比其他葡萄球菌生长更快，并且经常显示抗生素耐药性，因此我们寻找新的方法来控制皮肤微生物群落平衡，而不使用常规的抗微生物药物。方法：用扫描电镜详细观察了SA在体外形成生物膜的情况。大约500种物质进行了筛选SA生长和SA biofilm.Results的选择性影响：我们发现，木糖醇抑制糖萼的形成，法尼醇溶解纤维蛋白纤维。法尼醇仅抑制SA的生长，而不影响SE的生长。木糖醇和法尼醇协同抑制SA的生物膜形成。结论：木糖醇和法尼醇具有控制皮肤微生物平衡的潜力，因为它们具有选择性作用和抑制生物膜形成。它们可能提供一种有用且安全的方法来护理SA定植的皮肤，而无需使用抗生素。(c)2005年日本皮肤病研究学会。由Elsevier爱尔兰有限公司出版。保留所有权利。
Background: Staphylococcus aureus (SA) is usually present in atopic dry skin, and not only in regions seriously affected by atopic dermatitis. SA discharges various toxins and enzymes that injure the skin, and forms a biofilm from fibrin fiber and glycocalyx; the biofilm is important for adhesion of SA to the skin and for resistance to antimicrobial agents. Even highly effective moisturizers do not work perfectly on atopic dry skin. Staphylococcus epidermidis (SE) is a major constituent of skin microflora on healthy human skin, and provides protection against the growth of pathogenic bacteria.Objectives: Since treatment with anti-microbials may Lead to re-growth of SA, which grows faster than other Staphylococci and often shows antibiotic resistance, we searched for novel approaches to control the skin-microfloral balance without using conventional anti-microbials.Method: Biofilm formation by SA in vitro was observed in detail using scanning electron microscopy. Approximately 500 substances were screened for a selective effect on SA growth and SA biofilm.Results: We found that xylitol inhibited the formation of glycocalyx, and farnesol dissolved fibrin fibers. Farnesol suppressed the growth of only SA, and did not affect that of SE. Xylitol and farnesol synergistically inhibited biofilm formation by SA. Conclusion: Xylitol and farnesol have potential for controlling the skin-microfloral balance because of their selective effects and inhibition of biofilm formation. They might provide a useful and safe method to care for skin colonized by SA, without using antibiotics. (c) 2005 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.