Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins
Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins
复制标题
使用环糊精定量分析红细胞膜磷脂和调节细胞-巨噬细胞相互作用
DOI：
10.1038/s41598-020-72176-3
复制
发表时间：
2020
影响因子：
4.6
通讯作者：
Farnoud, Amir M.
中科院分区：
文献类型：
--
作者：
Vahedi, Amid;Bigdelou, Parnian;Farnoud, Amir M.
真核细胞的质膜在磷脂组成方面是不对称的。分析外小叶的脂质组成对了解健康和疾病中的细胞膜生物学具有重要意义。本文报道了一种基于环糊精介导的脂质交换来表征红细胞外小叶磷脂的方法。甲基-α-环糊精负载外源性脂质，从细胞膜外小叶提取磷脂，同时将脂质传递给细胞，维持细胞膜的完整性。薄层色谱和脂质组学分析表明，提取的脂质来源于膜外小叶。磷脂酰胆碱（PC）和鞘磷脂（SM）是红细胞外小叶中含量最多的磷脂，其中PC 34:1和SM 34:1含量最多。荧光猝灭证实外源性脂质传递到细胞外小叶。然后采用脂质交换法从衰老红细胞的外小叶中去除吞噬细胞识别标志物磷脂酰丝氨酸。与对照细胞相比，具有重组膜的衰老红细胞的吞噬量显著降低，这表明脂质交换过程的效率及其在调节细胞-细胞相互作用方面的应用。
The plasma membrane of eukaryotic cells is asymmetric with respect to its phospholipid composition. Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease. Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported. Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity. Thin layer chromatography and lipidomics demonstrated that the extracted lipids were from the membrane outer leaflet. Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species. Fluorescence quenching confirmed the delivery of exogenous lipids to the cell outer leaflet. The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs. Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell–cell interactions.
登录
查看更多内容
DOI：
--
发表时间：
2018
期刊：
Traffic : the International Journal of Intracellular Transport
影响因子：
--
作者：
T. Steck;Y. Lange
通讯作者：
Y. Lange
胆固醇与不饱和磷脂和鞘磷脂相互作用的热力学比较。
影响因子：
3.4
作者：
Alekos D Tsamaloukas;Halina Szadkowska;H. Heerklotz
通讯作者：
H. Heerklotz
14. 使用环糊精催化的脂质交换制备的不对称脂质囊泡的形成和性质
DOI：
--
发表时间：
2019
期刊：
Characterization of Biological Membranes
影响因子：
--
作者：
E. London
通讯作者：
E. London
芘磷脂/γ-环糊精复合物的特性
影响因子：
3.4
作者：
K. Tanhuanpää;K. Cheng;K. Anttonen;J. Virtanen;P. Somerharju
通讯作者：
P. Somerharju
DOI：
10.1111/pce.12649
发表时间：
2016-04
期刊：
Plant, cell & environment
影响因子：
--
作者：
Narayanan S;Tamura PJ;Roth MR;Prasad PV;Welti R
通讯作者：
Welti R