Evidence from mitochondrial DNA that head lice and body lice of humans (Phthiraptera: Pediculidae) are conspecific
Evidence from mitochondrial DNA that head lice and body lice of humans (Phthiraptera: Pediculidae) are conspecific
复制标题
DOI：
10.1603/0022-2585-39.4.662
复制
发表时间：
2002-07-01
影响因子：
2.1
通讯作者：
Barker, SC
中科院分区：
文献类型：
--
作者：
Leo, NP;Campbell, NJH;Barker, SC
人类头虱和体虱的具体地位已经争论了200多年。为了阐明头虱和体虱的具体状况，我们对来自9个国家的28头虱和28体虱的细胞色素氧化酶I（COI）基因的524个碱基对（bp）进行了测序。10个单倍型，不同的1-5 bp的II核苷酸的位置被确定。这些序列的同源性表明，这些头虱和体虱不是从单系谱系。事实上，头虱和体虱共有我们发现的10种单倍型中的3种。F-ST值和单倍型频率的精确检验显示头虱和体虱之间存在显著差异。然而，同样的测试也显示了来自不同国家的虱子之间的显着差异。事实上，单倍型频率的变化更多地是由来自不同国家的虱子之间的差异而不是头虱和体虱之间的差异来解释的。我们的研究结果表明：（1）珠虱和体虱不代表单系谱系，是同种的;（2）来自不同国家的虱子种群之间的基因流是有限的;（3）COI单倍型的频率可以用来研究头虱和体虱种群之间的母体基因流，从而研究虱子在人类宿主中的传播。
The specific status of the head and body lice of humans has been debated for more than 200 yr. To clarify the specific status of head and body lice, we sequenced 524 base pairs (bp) of the cytochrome oxidase I (COI) gene of 28 head and 28 body lice from nine countries. Ten haplotypes that differed by 1-5 bp at II nucleotide positions were identified. A phylogeny of these sequences indicates that these head and body lice are not from reciprocally monophyletic lineages. Indeed, head and body lice share three of the 10 haplotypes we found. F-ST values and exact tests of haplotype frequencies showed significant differences between head and body lice. However, the same tests also showed significant differences among lice from different countries. Indeed, more of the variation in haplotype frequencies was explained by differences among lice from different countries than by differences between head and body lice. Our results indicate the following: (1) bead and body lice do not represent reciprocally monophyletic lineages and are conspecific; (2) gene flow among populations of lice from different countries is limited; and (3) frequencies of COI haplotypes can be used to study maternal gene flow among populations of head and body lice and thus transmission of lice among their human hosts.