Critical role of tumor necrosis factor-α in the early process of wound healing in skin
Critical role of tumor necrosis factor-α in the early process of wound healing in skin
复制标题
DOI：
10.1016/j.jdds.2016.09.001
复制
发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Tachi, Masahiro
中科院分区：
文献类型：
--
作者：
Ritsu, Masae;Kawakami, Kazuyoshi;Tachi, Masahiro
肿瘤坏死因子-α被迅速释放并在伤口组织中引发炎症，但其在伤口愈合中的确切作用尚不完全清楚。我们利用皮肤全层创伤的小鼠模型，研究了这种细胞因子在早期愈合过程中的作用。肿瘤坏死因子-α的合成在创面形成后立即被检测到，在最初的几个小时内增加，在第一天达到峰值，然后下降到基础水平。在接受抗肿瘤坏死因子-α单抗治疗的小鼠中，与对照组相比，在第3天，伤口闭合显著延迟，肉瘤边缘之间的距离显著延长。在第3天，抗肿瘤坏死因子-α单抗治疗组小鼠的炎症细胞和成纤维细胞密度明显低于对照组。相反，当小鼠接受生物活性的肿瘤坏死因子-α治疗时，伤口愈合在第3天就加速了。这些结果表明，肿瘤坏死因子-α参与了伤口愈合的早期过程。(C)2016年提交人。爱思唯尔公司代表沙特国王大学制作和主办。
Tumor necrosis factor (TNF)-alpha is quickly released and initiates inflammation at wound tissues, but its precise role in wound healing is not fully understood. We examined the contribution of this cytokine to the early process of healing using a mouse model with fullthickness wounds in skin. TNF-alpha synthesis was detected just after wound creation, increased during the first several hours, reached a peak level on day 1, and then decreased to the basal level. In mice treated with anti-TNF-alpha mAb, wound closure was significantly delayed, and distances between the panniculus carnosus edges were significantly longer on day 3, compared with control. Inflammatory cell and fibroblast density were markedly decreased on day 3 in the anti-TNF-alpha mAb-treated mice compared with control. In contrast, wound healing was accelerated on day 3 when mice were treated with bioactive TNF-alpha. These results indicate that TNF-alpha is involved in the early process of wound healing. (C) 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.