The CsNPR1 gene expression modulation in citrus and understanding the defense mechanism against Huanglongbing by screening CsNPR1-interacting proteins
The CsNPR1 gene expression modulation in citrus and understanding the defense mechanism against Huanglongbing by screening CsNPR1-interacting proteins
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DOI：
10.1016/j.scienta.2021.110375
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发表时间：
2021-10
影响因子：
4.3
通讯作者：
Qia-qing Wu;M. Moniruzzaman;Huanxue Yan;Yuanda Lv;Jiang Bo;Jiang Nonghui;Y. Zhong
中科院分区：
文献类型：
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作者：
Qia-qing Wu;M. Moniruzzaman;Huanxue Yan;Yuanda Lv;Jiang Bo;Jiang Nonghui;Y. Zhong
黄龙病（HLB）病又称柑橘绿化病，是柑橘类水果种植业的严重威胁。迄今为止，尚未开发出任何有效的根治HLB的方法，也没有开发出抗HLB的品种。本研究旨在为揭示柑橘响应HLB的分子调控机制提供线索，为抗HLB品种选育提供参考基因。用不同的激素和逆境胁迫处理沙田柚 (Citrus grandis(L.) Osbeck) 一岁植株。我们发现抗病基因CsNPR1的表达受到赤霉素、吲哚-3-乙酸和水杨酸的调节，而在干旱、盐和机械损伤等不利胁迫下也会发生显着的表达变化。此外，我们还增强了柑橘中 CsNPR1 的表达并评估了植物对 HLB 的抗性。最后，为了研究 CsNPR1 相互作用蛋白，构建了高质量的柑橘 cDNA 文库。通过酵母双杂交（Y2H）和双分子荧光互补（BiFC）测定来筛选和验证相互作用的蛋白质。筛选并验证了两种 CsNPR1 相互作用蛋白（CsHSPRO2 和 CsHIR2）。结果表明，过表达CsNPR1的转基因植物表现出增强的HLB耐受性。因此，CsNPR1 通过与 CsHSPRO2 或 CsHIR2 蛋白相互作用，在柑橘的应激和疾病防御中发挥作用。
Huanglongbing (HLB) disease, also known as citrus greening, is a severe threat for citrus fruit cultivation industry. Till date, neither any effective radical method to control HLB nor HLB resistant cultivar is developed. The aim of this study was to provide clues for unraveling the molecular regulatory mechanism of citrus in response to HLB, and to provide reference gene for breeding of HLB resistant cultivar. One-year-old plants of Shatian pomelo (Citrus grandis(L.) Osbeck) were treated with different hormones and adverse stresses. We found the disease resistance gene,CsNPR1,expression was modulated by gibberellin, indole-3-acetic acid, and salicylic acid, whereas significant expression changes also occurred under adverse stresses including drought, salt, and mechanical injury. Additionally, we enhanced the expression ofCsNPR1in citrus and evaluated plant resistance against HLB. Finally, to investigate CsNPR1 interacting proteins, a high-quality citrus cDNA library was constructed. The interacting proteins were screened and verified by yeast two-hybrid (Y2H) and Bimolecular Fluorescence Complementation (BiFC) assay. Two CsNPR1-interacting proteins (CsHSPRO2 and CsHIR2) were screened and verified. The results showed that the transgenic plants overexpressingCsNPR1exhibited enhance tolerance against HLB. Thus, CsNPR1 plays a role in stress and disease defense in citrus through interaction with CsHSPRO2 or CsHIR2 proteins.