A cross-sectional epidemiological survey of bovine trypanosomosis and its vectors in the Savelugu and West Mamprusi districts of northern Ghana
A cross-sectional epidemiological survey of bovine trypanosomosis and its vectors in the Savelugu and West Mamprusi districts of northern Ghana
复制标题
DOI：
10.1016/j.vetpar.2004.03.009
复制
发表时间：
2004-06-10
影响因子：
2.6
通讯作者：
Geerts, S
中科院分区：
文献类型：
--
作者：
Mahama, CI;Desquesnes, M;Geerts, S
在加纳北部两个土地利用和环境特征不同的地区（Savelugu和West Mamprusi）调查了牛锥虫病的流行病学。沙韦卢古区土地利用强度和环境变化疑似较高。沿白伏特河及其支流进行的横断面昆虫学调查证实，冈比亚绿盲蝽（Glossina palpalis gambiensis）和tachinoides的存在。以采采蝇密度与采采蝇感染率之积测量的攻毒指数，西Mamprusi区（19.6）远高于Savelugu区（4.7）。从随机选择的萨韦卢古地区16个村庄和西曼普西地区13个村庄共放养了1013头牛（萨韦卢古508头，西曼普西505头）。血标本采用褐皮虫技术（BCT）检测锥虫。BCT呈阳性或BCT呈阴性但堆积细胞体积（PCV）值低于21的血样进一步用聚合酶链反应检测锥虫DNA。用间接ELISA法检测所有牛的血浆样本的锥虫抗体。牛锥虫病的寄生虫学和血清学流行率在西马姆普西区（分别为16%和53%）显著高于萨韦卢古区（分别为8%和24%）。利用PCV作为贫血指标，对村畜群一级的动物健康状况进行了评估，提供了整个研究地区流行的各种流行病学情景。(C) 2004 Elsevier B.V.版权所有
The epidemiology of bovine trypanosomosis was investigated in two districts (Savelugu and West Mamprusi) of Northern Ghana with different land use and environmental characteristics. The land use intensity and environmental change was suspected to be higher in the Savelugu District. A cross-sectional entomological survey conducted along the White Volta river and its tributaries confirmed the presence of only Glossina palpalis gambiensis and G. tachinoides. The challenge index as measured by the product of tsetse density and tsetse infection rate was much higher in the West Mamprusi (19.6) than in the Savelugu district (4.7). A total of 10 13 cattle (508 in Savelugu and 505 in West Mamprusi) were bled from a random selection of 16 villages in the Savelugu District and 13 villaaes in the West Mamprusi District. Blood samples were examined for trypanosomes by the buffy coat technique (BCT). Blood samples that were positive in the BCT or negative in the BCT but with packed cell volume (PCV) values below 21 were further tested with a polymerase chain reaction for trypanosomal DNA. Plasma samples of all cattle were serologically tested with an indirect ELISA for trypanosomal antibodies. The parasitological and serological prevalence of bovine trypanosomoses was significantly higher in West Mamprusi (16 and 53%, respectively) than in Savelugu District (8 and 24%, respectively). An evaluation of animal health at the village herd level, using PCV as an index of anaemia, provided various epidemiological scenarios prevalent in the entire study-area. (C) 2004 Elsevier B.V. All rights reserved.