Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1.
Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1.
复制标题
开发特异性阻断与 LRP1 相互作用的单克隆抗 ADAMTS-5 抗体。
DOI：
10.1080/19420862.2017.1304341
复制
发表时间：
2017
期刊：
影响因子：
5.3
通讯作者：
Yamamoto,Kazuhiro
中科院分区：
文献类型：
--
作者：
Santamaria,Salvatore;Fedorov,Oleg;McCafferty,John;Murphy,Gillian;Dudhia,Jayesh;Nagase,Hideaki;Yamamoto,Kazuhiro
强效的聚集酶ADAMTS-5由软骨细胞组成性分泌，但在正常软骨细胞表面的内吞受体LRP1中被迅速内吞。因此很难检测产生的总ADAMTS-5活性。在这项研究中，我们分离了一种单克隆抗adamts -5抗体1B7，它可以阻断lrp1介导的内化，而不影响其溶聚活性。将1B7添加到培养的人软骨细胞中，发现细胞产生的ADAMTS-5具有充分的聚解活性。1B7是估计ADAMTS-5活性和确定其在组织中的潜在作用的有用工具。
The potent aggrecanase ADAMTS-5 is constitutively secreted by chondrocytes, but it is rapidly endocytosed in normal cartilageviathe cell surface endocytic receptor LRP1. Therefore it is difficult to detect the total ADAMTS-5 activity produced. In this study, we isolated a monoclonal anti-ADAMTS-5 antibody 1B7 that blocks LRP1-mediated internalization without affecting the aggrecanolytic activity. Addition of 1B7 to cultured human chondrocytes revealed the full aggrecanolytic activity of ADAMTS-5 generated by the cells. 1B7 is a useful tool to estimate the ADAMTS-5 activity and to identify its potential roles in the tissues.