Yiwei decoction promotes apoptosis of gastric cancer cells through spleen-derived exosomes.
Yiwei decoction promotes apoptosis of gastric cancer cells through spleen-derived exosomes.
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益胃汤通过脾源性exosomes促进胃癌细胞凋亡。
DOI：
10.3389/fphar.2023.1144955
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
益胃汤（YWD）是一种临床有效的预防和治疗胃癌复发转移的中药配方。根据中医理论，冬青补益机体，增强机体对胃癌复发和转移的抵抗力，可能是通过对脾脏的免疫调节。本研究旨在探讨黄芪多糖对大鼠脾源性外泌体是否具有抑制肿瘤细胞增殖的作用，阐明黄芪多糖的抗癌作用，为黄芪多糖作为一种新的胃癌临床治疗方法提供依据。脾源性外泌体通过超离心获得，并通过透射电镜、纳米颗粒跟踪分析和western blot分析进行鉴定。然后用免疫荧光染色确定肿瘤细胞中外泌体的位置。用不同浓度的外泌体处理肿瘤细胞后，通过细胞计数试剂盒8 （CCK8）和集落形成实验检测外泌体对细胞增殖的影响。流式细胞术检测肿瘤细胞凋亡。颗粒分析和western blot鉴定脾组织上清提取的物质为外泌体。免疫荧光染色显示，脾源性外泌体被HGC-27细胞吞噬，CCK8检测证实，在30 μg/mL浓度下，ypd处理的脾源性外泌体相对肿瘤抑制率达到70.78% （p < 0.05）。与30 μg/mL浓度的对照外泌体相比，集落形成实验显示，30 μg/mL浓度的ydd处理脾脏外泌体集落数量减少了99.03% （p < 0.01）。此外，流式细胞术分析显示，30 μg/mL的ywd处理外泌体使细胞凋亡率提高至43.27%，显著高于30 μg/mL的对照组（25.91%）（p < 0.05）。综上所述，YWD处理动物脾源性外泌体通过诱导凋亡抑制HGC-27细胞的增殖，提示脾源性外泌体参与了YWD抗肿瘤作用的介导。这些结果证明了黄芪多糖作为中药方剂具有一种新的外泌体介导的抗癌作用，从而支持使用黄芪多糖处理的外泌体作为临床治疗胃癌的新途径。
Yiwei decoction (YWD) is a formula of traditional Chinese medicine (TCM) that is clinically effective for the prevention and treatment of gastric cancer recurrence and metastasis. According to the theory of TCM, YWD tonifies the body and strengthens the body’s resistance to gastric cancer recurrence and metastasis potentially via the immune regulation of the spleen. The aims of the present study were to investigate whether YWD-treated spleen-derived exosomes in rats inhibit the proliferation of tumor cells, to elucidate the anticancer effects of YWD, and to provide evidence supporting the use of YWD as a new clinical treatment for gastric cancer. Spleen-derived exosomes were obtained by ultracentrifugation and identified by transmission electron microscopy, nanoparticle tracking analysis, and western blot analysis. The location of the exosomes in tumor cells was then determined by immunofluorescence staining. After tumor cells were treated with different concentrations of exosomes, the effect of exosomes on cell proliferation was determined by cell counting kit 8 (CCK8) and colony formation assays. Tumor cell apoptosis was detected by flow cytometry. Particle analysis and western blot analysis identified the material extracted from spleen tissue supernatant as exosomes. Immunofluorescence staining showed that spleen-derived exosomes were taken up by HGC-27 cells, and the CCK8 assay confirmed that the relative tumor inhibition rate of YWD-treated spleen-derived exosomes in the 30 μg/mL reached 70.78% compared to control exosomes in the 30 μg/mL (p < 0.05). Compared to control exosomes in the 30 μg/mL, the colony formation assay indicated that YWD-treated spleen-derived exosomes in the 30 μg/mL colonies have decreased by 99.03% (p < 0.01). Moreover, flow cytometry analysis showed that treatment with YWD-treated exosomes in the 30 μg/mL increased the apoptosis rate to 43.27%, which was significantly higher than that of the control group in the 30 μg/mL (25.91%) (p < 0.05). In conclusion, spleen-derived exosomes from YWD-treated animals inhibit the proliferation of HGC-27 cells via inducing apoptosis, suggesting that spleen-derived exosomes are involved in mediating the antitumor effect of YWD. These results demonstrated a novel exosome-mediated anticancer effect of YWD as a TCM formula, thereby supporting the use of YWD-treated exosomes as a new approach for the clinical treatment of gastric cancer.
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影响因子：
4.9
作者：
通讯作者：
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源自 miR-122 修饰的脂肪组织来源的 MSC 的外泌体可增加肝细胞癌的化疗敏感性
影响因子：
28.5
作者：
Lou G;Song X;Yang F;Wu S;Wang J;Chen Z;Liu Y
通讯作者：
Liu Y
影响因子：
4
作者：
Jia, Jianjun;Guo, Suiqun;Xie, Xingmei
通讯作者：
Xie, Xingmei
影响因子：
4.6
作者：
Lee, Seong-Ho;Cekanova, Maria;Baek, Seung Joon
通讯作者：
Baek, Seung Joon
影响因子：
64.5
作者：
Jeppesen, Dennis K.;Fenix, Aidan M.;Coffey, Robert J.
通讯作者：
Coffey, Robert J.