UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal Injury
UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal Injury
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基于 UPLC/MS 的代谢组学研究氢气吸入对草酸钙肾损伤小鼠的保护作用
DOI：
10.1248/bpb.b18-00307
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发表时间：
2018-11-01
影响因子：
2
通讯作者：
Guo, Zhiyong
中科院分区：
文献类型：
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作者：
Lu, Hongtao;Ding, Jiarong;Guo, Zhiyong
氢对酒石酸钙诱导的肾损伤具有显著的保护作用，但其对代谢特征的影响尚不清楚。本研究显示了氢对肾损伤模型中血清和尿液代谢物的影响。使用基于超高效液相色谱四极杆飞行时间-质谱的代谢组学来确定代谢变化。25种血清代谢物和14种尿液代谢物在氮氧吸入（NO）、氮氧吸入联合草酸钙诱导（CaOx）和氢吸入联合草酸钙诱导（HO+CaOx）组中存在差异。19种血清代谢物和7种尿液代谢物在氢气（112）处理后显著恢复至正常水平。这些代谢产物主要与氨基酸代谢、脂肪酸代谢和磷脂代谢有关。这项研究表明，一个全面的代谢组学方法是一个有效的策略，以阐明氢处理的影响，对草酸钙诱导的肾损伤的机制。
Hydrogen has a significant protective effect on calcium oxalate-induced renal injury, but its effect on metabolic profiles is unknown. This study showed the effects of hydrogen on serum and urine metabolites in a renal injury model. Ultra-HPLC quadrupole time-of-flight-MS-based metabolomics was used to characterise metabolic variations. Twenty-five serum metabolites and 14 urine metabolites showed differences in the the nitrogen and oxygen inhalation (NO), nitrogen and oxygen inhalation combined with calcium oxalate induction (CaOx), and hydrogen inhalation combined with calcium oxalate induction (HO+CaOx) groups. Nineteen serum metabolites and 7 urine metabolites showed significant restoration to normal levels after hydrogen gas (112) treatment. These metabolites are primarily related to amino acid metabolism, fatty acid metabolism, and phospholipid metabolism. This study showed that a comprehensive metabolomics approach is an effective strategy to elucidate the mechanisms underlying the effects of hydrogen treatment on calcium oxalate-induced renal injury.