Pou5f1 Transcription Factor Controls Zygotic Gene Activation In Vertebrates
Pou5f1 Transcription Factor Controls Zygotic Gene Activation In Vertebrates
复制标题
DOI：
10.1126/science.1242527
复制
发表时间：
2013-08-30
期刊：
影响因子：
56.9
通讯作者：
Onichtchouk, Daria
中科院分区：
文献类型：
--
作者：
Leichsenring, Manuel;Maes, Julia;Onichtchouk, Daria
多细胞动物的发育最初是由储存在卵母细胞中的母体基因产物控制的。在母体到合子的转变过程中，合子基因的转录开始，发育控制开始受到合子基因产物的调控。在果蝇中，转录因子塞尔达特异性地与最早的合子基因的启动子结合，并使它们激活。目前尚不清楚其他动物是否存在类似的规则。我们发现，斑马鱼Pou 5 f1，同源的哺乳动物多能性转录因子Oct 4，占据SOX-POU结合位点的合子转录开始之前，激活最早的合子基因。我们的数据位置Pou 5 f1和SOX-POU网站在脊椎动物合子基因激活网络的中心，并提供合子基因激活和多能性控制之间的联系。
The development of multicellular animals is initially controlled by maternal gene products deposited in the oocyte. During the maternal-to-zygotic transition, transcription of zygotic genes commences, and developmental control starts to be regulated by zygotic gene products. In Drosophila, the transcription factor Zelda specifically binds to promoters of the earliest zygotic genes and primes them for activation. It is unknown whether a similar regulation exists in other animals. We found that zebrafish Pou5f1, a homolog of the mammalian pluripotency transcription factor Oct4, occupies SOX-POU binding sites before the onset of zygotic transcription and activates the earliest zygotic genes. Our data position Pou5f1 and SOX-POU sites at the center of the zygotic gene activation network of vertebrates and provide a link between zygotic gene activation and pluripotency control.