Metallo-β-lactamase-producing Pseudomonas putida as a reservoir of multidrug resistance elements that can be transferred to successful Pseudomonas aeruginosa clones
Metallo-β-lactamase-producing Pseudomonas putida as a reservoir of multidrug resistance elements that can be transferred to successful Pseudomonas aeruginosa clones
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DOI：
10.1093/jac/dkp491
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发表时间：
2010-03-01
影响因子：
5.2
通讯作者：
Oliver, Antonio
中科院分区：
文献类型：
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作者：
Juan, Carlos;Zamorano, Laura;Oliver, Antonio
研究2005 ~ 2008年西班牙某医院分离的产金属β-内酰胺酶（MBL）铜绿假单胞菌和恶臭假单胞菌的流行病学、致病性、相关基因及流行病学特征。PFGE和多位点序列分型（MLST）的克隆相关性进行了评估。通过限制性片段长度多态性、Southern印迹和电穿孔对携带MBL的质粒进行表征。通过克隆和测序研究了MBL遗传元件。在8名患者中检测到产MBL的恶臭假单胞菌（各1个克隆; 2个携带bla（Vim-1）和6个携带bla（Vim-2）），占恶臭假单胞菌/荧光菌组所有感染的14%。在同一时期，仅在0.3%的铜绿假单胞菌感染（11例患者）中检测到MBL。PFGE显示了4个铜绿假单胞菌克隆：1个产生bla（Vim-13）（2例患者）; 3个产生bla（Vim-2）（分别为2例患者、6例患者和1例患者）。MLST表明，Vim-13克隆是国际传播的序列类型（ST）235，而主要的Vim-2谱系对应于与慢性呼吸道感染相关的ST 179。Vim-1整合子显示具有质粒和染色体定位，而Vim-13整合子仅为染色体。Vim-2整合子位于铜绿假单胞菌和恶臭假单胞菌的同一个转座子（Tn 402/Tn 5053-like）上，提示VIM-2整合子在VIM-2传播中起重要作用。这些多药耐药元件传播到成功的铜绿假单胞菌克隆提出了一个主要的流行病学和临床威胁。
To study the prevalence, nature, involved genetic elements and epidemiology of metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and Pseudomonas putida isolated in a Spanish hospital between 2005 and 2008.Etests were used for susceptibility testing and screening for MBLs, confirmed through bla(VIM) PCRs and sequencing. Clonal relatedness was evaluated by PFGE and multilocus sequence typing (MLST). MBL-carrying plasmids were characterized by restriction fragment length polymorphism, Southern blot and electroporation. MBL genetic elements were studied by cloning and sequencing.MBL-producing P. putida was detected in eight patients (one clone each; two harbouring bla(VIM-1) and six harbouring bla(VIM-2)), representing 14% of all the infections by the P. putida/fluorescens group. MBLs were detected in only 0.3% of P. aeruginosa infections (11 patients) during the same period. PFGE revealed four P. aeruginosa clones: one producing bla(VIM-13) (two patients); and three producing bla(VIM-2) (two patients, six patients and one patient, respectively). MLST indicated that the VIM-13 clone was the internationally spread sequence type (ST)235, while the major VIM-2 lineage corresponded to ST179, which is associated with chronic respiratory infections. The VIM-1 integron was shown to have both plasmid and chromosomal location, while the VIM-13 integron was only chromosomal. The VIM-2 integron was located in the same transposon (Tn402/Tn5053-like) in all P. aeruginosa and P. putida isolates, suggesting its crucial role in the dissemination of VIM-2.The high diversity and proportion of MBL-positive P. putida suggests an environmental reservoir of these resistance determinants. Dissemination of these multidrug resistance elements to successful P. aeruginosa clones presents a major epidemiological and clinical threat.