A Shift from Colon- to Ileum-Predominant Bacteria in Ileal-Pouch Feces Following Total Proctocolectomy
A Shift from Colon- to Ileum-Predominant Bacteria in Ileal-Pouch Feces Following Total Proctocolectomy
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DOI：
10.1007/s10620-012-2165-9
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发表时间：
2012-11-01
影响因子：
3.1
通讯作者：
Fukushima, Kouhei
中科院分区：
文献类型：
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作者：
Hinata, Manami;Kohyama, Atsushi;Fukushima, Kouhei
我们之前使用末端限制性片段多态性分析研究了全直肠结肠切除术后造口袋的粪便菌群。尽管聚类分析的结果清楚地表明，细菌种群（包括产生 213 bp PCR 片段的不明细菌）向造口袋中的结肠样群落移动，但它没有追踪粪便细菌个体物种的变化。本研究的目的是估计回肠造口术后不同时间的造口袋粪便样本中 10 种细菌物种、簇、群或亚群（包括先前研究中产生 213 bp 片段的细菌）的基因组拷贝数共采集溃疡性结肠炎术后患者以及健康志愿者的粪便样本117份。我们使用 16S rRNA 基因的实时聚合酶链式反应来估计 9 个细菌群体的基因组拷贝数以及通过 DNA 测序鉴定后产生 213 bp 片段的细菌。我们证明了造口闭合后直肠结肠切除术患者中厌氧菌和结肠优势菌（如球状梭菌、薄梭菌、脆弱拟杆菌和 Atopobium）数量呈时间依赖性增加。相比之下，回肠主要细菌种类（如乳酸菌和粪肠球菌）的数量下降。我们的数据证实了之前的研究结果，即全直肠结肠切除术后育儿袋中的粪便菌群发生了显着变化，并进一步证明回肠细菌的数量和多样性减少，同时形成了更像结肠的群落。目前的数据对于未来分析回肠袋的病理状况至关重要。
We previously investigated fecal flora of the pouch after total proctocolectomy using terminal restriction fragment polymorphism analysis. Although the results of the cluster analysis demonstrated clearly that bacterial populations, including an unidentified bacteria generating a 213-bp PCR fragment, moved toward a colon-like community in the pouch, it did not track changes in the individual species of fecal bacteria.The aim of the present study was to estimate genome copy number of ten bacterial species, clusters, groups, or subgroups (including the bacteria generating 213-bp fragment in the previous study) in feces samples from pouches at various times following ileostomy closure.A total of 117 stool samples were collected from patients with ulcerative colitis after surgery as well as healthy volunteers. We used real-time polymerase chain reaction of the 16S rRNA gene to estimate genome copy numbers for the nine bacterial populations and the bacteria generating 213-bp fragment after identification by DNA sequencing.We demonstrated a time-dependent increase in the number of anaerobic and colon-predominant bacteria (such as Clostridium coccoides, C. leptum, Bacteroides fragilis and Atopobium) present in proctocolectomy patients after stoma closure. In contrast, numbers of ileum-predominant bacterial species (such as Lactobacillus and Enterococcus faecalis) declined.Our data confirm previous findings that fecal flora in the pouch after total proctocolectomy changes significantly, and further demonstrate that the number and diversity of ileal bacteria decreases while a more colon-like community develops. The present data are essential for the future analysis of pathological conditions in the ileal pouch.