EFFECTS OF CHICKEN ANEMIA VIRUS ON CELL-MEDIATED IMMUNE FUNCTION IN CHICKENS EXPOSED TO THE VIRUS BY A NATURAL ROUTE
EFFECTS OF CHICKEN ANEMIA VIRUS ON CELL-MEDIATED IMMUNE FUNCTION IN CHICKENS EXPOSED TO THE VIRUS BY A NATURAL ROUTE
复制标题
DOI：
10.2307/1591660
复制
发表时间：
1993-04-01
期刊：
影响因子：
1.4
通讯作者：
MCNULTY, MS
中科院分区：
文献类型：
--
作者：
MCCONNELL, CDG;ADAIR, BM;MCNULTY, MS
用CAV经口接种3周龄雏鸡，并在接种后不同时间（PI）处死。取出脾脏，用刀豆素A刺激细胞，测定淋巴细胞转化反应。还测定了来自这些培养物的上清液的T细胞生长因子（TCGF）和干扰素。免疫鸡脾脏和骨髓的巨噬细胞检测白细胞介素-1（IL-1）的产生、Fc受体的表达、吞噬功能和杀菌活性。对照在整个实验中保持CAV抗体阴性。CAV接种鸡的淋巴细胞转化反应和TCGF和干扰素的产生与对照组有显着差异。在Pl第14、21和28天差异最大。还观察到脾巨噬细胞产生白细胞介素-1，以及骨髓巨噬细胞的Fc受体表达，吞噬作用和杀菌活性的显着差异。
Three-week-old chicks were inoculated orally with CAV and killed at various times postinoculation (PI). The spleens were removed, the cells were stimulated with concanavalan A, and lymphocyte transformation responses were determined. Supernatants from these cultures were also assayed for T-cell growth factor (TCGF) and interferon. Adherent macrophages from spleen or bone marrow were assayed for interleukin-1 production, Fc receptor expression, phagocytosis, and bactericidal activity, All CAV-inoculated chickens developed CAV antibodies, but no anemia was seen. Controls remained CAV-antibody-negative throughout the experiment. CAV-inoculated chickens showed significant differences from controls in their lymphocyte transformation responses and in production of TCGF and interferon. Differences were greatest at 14, 21, and 28 days Pl. Significant differences were also observed in interleukin-1 production by spleen macrophages, as well as in Fc receptor expression, phagocytosis, and bactericidal activity of bone-marrow macrophages.