The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
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DOI：
10.1093/clinchem/hvaa125
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发表时间：
2020-08-01
影响因子：
9.3
通讯作者：
Vandesompele, Jo
中科院分区：
文献类型：
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作者：
Huggett, Jim F.;Whale, Alexandra S.;Vandesompele, Jo
数字PCR （dPCR）自2013年发布《数字PCR实验出版最低信息》（dMIQE）指南以来，随着仪器、软件、应用和我们对其技术潜力的理解的进步，已经有了很大的发展。然而，这些发展也带来了相关的挑战；数据分析步骤，包括阈值设置，可能是困难的，纯化、浓缩和修饰核酸所需的分析前步骤可能导致测量误差。为了协助结论的独立确证，需要全面披露所有相关的实验细节。为了支持社区并反映dPCR的日益增长的使用，我们提出了dMIQE的更新，dMIQE2020，包括简化的dMIQE表格式，以帮助研究人员提供关键的实验信息和理解相关的实验过程。科学界采用dMIQE2020将有助于标准化实验方案，最大限度地有效利用资源，并进一步增强这一强大技术的影响。
Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.