CELL-MEDIATED-IMMUNITY ASSESSED BY SKIN TESTING (MULTITEST) .1. NORMAL VALUES IN HEALTHY DANISH ADULTS
CELL-MEDIATED-IMMUNITY ASSESSED BY SKIN TESTING (MULTITEST) .1. NORMAL VALUES IN HEALTHY DANISH ADULTS
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DOI：
10.1111/j.1398-9995.1987.tb00389.x
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发表时间：
1987-11-01
期刊：
影响因子：
12.4
通讯作者：
MOSBECH, H
中科院分区：
文献类型：
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作者：
MOESGAARD, F;NIELSEN, ML;MOSBECH, H
多重测试CMI系统由一个一次性多重穿刺器组成，该装置同时应用7个标准化的Recall抗原来评估细胞介导的免疫(CMI)。这7种抗原包括破伤风杆菌类毒素和白喉棒状杆菌类毒素、结核菌素和链球菌(C组)、白色念珠菌、须毛霉菌和奇异变形杆菌的抗原。对352名年龄在17岁到90岁之间的健康丹麦成年人进行了测试，以确定迟发性超敏反应(DTH)的发生率和大小。除6名健康成年人(98%)外，所有人对一种或多种抗原都有反应，阳性反应的中位数男性为4人，女性为3人。阳性反应率从结核菌素的91%到毛霉菌的11%不等。积极反应的数量随着年龄的增长而下降，女性的反应速度略快于男性。在65岁以上的老年人中，七种抗原中有六种的应答率明显较低，唯一的例外是毛滴虫，而七种抗原中的四种在女性中明显低于男性。当与年龄和性别相关时，除了男性对结核菌素的反应较大外，在对这七种抗原的反应大小方面没有发现重大差异。基于积极反应的数量和大小的评分系统显示出与年龄和性别相关的显著差异。17-65岁男性和女性的中位数分别为17 mm和14 mm，而65岁以上的中位数分别为13 mm和8 mm(P<；两项比较均为0.001)。
The Multitest CMI system consists of a disposable multiple puncture device that simultaneously applies seven standardized recall antigens for assessment of cell-mediated immunity (CMI). The seven antigens included were toxoid from Clostridium tetani and Corynebacterium diphteriae, tuberculin, plus antigens from streptococcus (group C), Candida albicans, Trichophyton mentagrophytes, and Proteus mirabilis. A population of 352 healthy Danish adults, aged between 17 and 90 years, was tested to determine the incidence and size of delayed-type hypersensitivity (DTH) responses. All but six healthy adults (98%) responded to one or more antigens, the median number of positive responses being four in males and three in females. The incidence of positive responses ranged from 91% for tuberculin to 11% for trichophyton. The number of positive responses declined with age, being somewhat faster in females than males. Six of the seven antigen response rates were significantly lower in the over 65-years-olds, the only exception being trichophyton, and for four of the seven antigens significantly lower in females compared to males. When correlated to age and sex no major differences were found with regard to the size of response to the seven antigens except that the tuberculin response was larger in males. A scoring system based on both number and size of positive responses revealed significant age and sex related differences. The median "score" in 17-65-year-old males and females were, respectively, 17 mm and 14 mm compared to 13 mm and 8 mm in those over 65 years old (P < 0.001 for both comparisons).