Serological Reactivity to Borrelia burgdorferi Sensu Lato in Dogs and Horses from Distinct Areas in Romania
Serological Reactivity to Borrelia burgdorferi Sensu Lato in Dogs and Horses from Distinct Areas in Romania
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DOI：
10.1089/vbz.2010.0254
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发表时间：
2011-09-01
影响因子：
2.1
通讯作者：
Spinu, Marina
中科院分区：
文献类型：
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作者：
Kiss, Timea;Cadar, Daniel;Spinu, Marina
莱姆病是宿主、媒介和媒介传播细菌之间复杂关系的完美模型。罗马尼亚的狗和马都可能受到感染。本研究的目的是评估罗马尼亚不同地区的狗和马对伯氏疏螺旋体的血清反应性。分别通过 ELISA 和 IFA 分析了罗马尼亚不同地区的 276 份狗样本和 260 份马样本。使用 Fisher 精确检验 (R 2.12.0) 评估了可能影响血清反应性的几个因素（地点、年龄、性别、职业和媒介暴露风险）的影响。狗体内抗疏螺旋体抗体的总体患病率为 6.52% (18/276)，中部地区的阳性率明显更高 (46.15%, 6/13, p = 0.0005)。血清反应性与职业相关，工作犬接触的程度更高。结果可能表明莱姆疏螺旋体病灶仅限于小区域，但需要对蜱种群中疏螺旋体流行率进行进一步研究来证实这一假设。在马中，观察到的全球血清阳性率为 11.92% (31/260)。未发现阳性结果与年龄、性别、县或职业之间存在相关性。这是对罗马尼亚狗和马中伯氏疏螺旋体抗体的首次血清学调查。
Lyme disease is a perfect model of the complex relationship between host, vector, and the vector-borne bacteria. Both dogs and horses in Romania are exposed to infection. The aim of the present study was to assess the seroreactivity against Borrelia burgdorferi sensu lato in dogs and horses from different regions of Romania. 276 samples from dogs and 260 samples from horses located in different regions of Romania were analyzed by ELISA and IFA, respectively. The effect of several factors potentially affecting seroreactivity (location, age, gender, occupation, and vector exposition risk) was evaluated using Fisher's exact test (R 2.12.0). The overall prevalence of anti-Borrelia antibodies was 6.52% (18/276) in dogs, with a significantly higher positivity (46.15%, 6/13, p = 0.0005) recorded in a midcountry region. Seroreactivity was correlated with occupation, with working dogs being more exposed. The results may indicate that Lyme borreliosis foci are restricted to small areas, but further studies on Borrelia prevalence in tick populations are needed to confirm this hypothesis. In horses, a global seroprevalence of 11.92% (31/260) was observed. No correlations were found between positive results and age, sex, county, or occupation. This is the first serological survey on antibodies to B. burgdorferi sensu lato in Romanian dogs and horses.