Expression of various matrix proteases and ets family transcriptional factors in ovarian cancer cell lines: Correlation to invasive potential
Expression of various matrix proteases and ets family transcriptional factors in ovarian cancer cell lines: Correlation to invasive potential
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DOI：
10.1006/gyno.2000.5944
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发表时间：
2000-11-01
影响因子：
4.7
通讯作者：
Kudo, R
中科院分区：
文献类型：
--
作者：
Nishikawa, A;Iwasaki, M;Kudo, R
目标。本研究的目的是确定与卵巢癌细胞侵袭活性有关的重要分子。我们比较了5种卵巢癌细胞系的生物学特性，即生长速度、运动能力和侵袭活性，并比较了各种基质蛋白(基质金属蛋白酶-1、基质金属蛋白酶-2、基质金属蛋白酶-9、膜型基质金属蛋白酶-1、基质金属蛋白酶-2、基质金属蛋白酶-9、膜型基质金属蛋白酶-1、基质金属蛋白酶-2、基质金属蛋白酶-3-基质金属蛋白酶、尿激酶型纤溶酶原激活物[uPA])及其抑制物(金属蛋白酶组织抑制物1[TIMP-1]、TIMP-2、纤溶酶原激活物抑制物1、[PAI-1]和PAI-2)及其潜在的转录调节因子E1AF和ETS-1的基因表达。结果。卵巢癌细胞的生长速度、运动能力和侵袭性之间没有明显的相关性，这表明卵巢癌细胞具有独立的恶性潜能。然而，HTBOA是一种低分化的癌细胞株，表现出高度侵袭活性、快速生长和增强的运动性。该细胞系还表达Ets转录因子E1AF和Ets-1，以及许多基质降解酶。3株表达E1AF的细胞生长迅速。高侵袭性细胞株HTBOA和HTOA(高分化浆液性囊腺癌)均可产生基质金属蛋白酶-2或基质金属蛋白酶-1，两种细胞系均表达MT1-基质金属蛋白酶和uPA。此外，用酶谱方法检测到hTBOA和hTOA中存在前基质金属蛋白酶-2和原-基质金属蛋白酶-1的活性形式。我们认为，活化的基质金属蛋白酶-2和基质金属蛋白酶-1在这些卵巢癌细胞的侵袭活性中起重要作用。(C)2000年学术出版社。
Objectives. The aim of our study was to determine the important molecules responsible for the invasive activity of ovarian cancer cells.Methods. We compared the biological characteristics, that is, growth rate, motility, and invasive activity, of five ovarian cancer cell Lines with the gene expression of various matrix proteases (matrix metalloproteinase-1 [MMP-1], MMP-2, MMP-9, membrane-type MMP type 1 [MT1-MMP], MT2-MMP, MT3-MMP, urokinase plasminogen activator [uPA]), their inhibitors (tissue inhibitor of metalloproteinase type 1 [TIMP-1], TIMP-2, plasminogen activator inhibitor type 1, [PAI-1], and PAI-2), and the potential transcriptional regulators E1AF and Ets-1. Results. There was no clear correlation in the growth rate, motility, and invasion, suggesting that there are independent properties for malignant potential in ovarian cancer cells. However, HTBOA, a poorly differentiated cancer cell line, exhibited highly invasive activity, rapid growth, and increased motility. This cell line also expressed both Ets transcriptional factors, E1AF and Ets-1, and many matrix-degrading enzymes. Three cell lines that expressed E1AF showed rapid cell growth. The highly invasive cell lines, HTBOA and HTOA (well-differentiated serous cystadenocarcinoma), produced either MMP-2 or MMP-1, and both cell lines expressed MT1-MMP and uPA. Furthermore, the active forms of pro-MMP-2 and pro-MMP-1 were detected in HTBOA and HTOA by zymography.Conclusion. We conclude that activated MMP-2 and MMP-1 are important in the invasive activity of these ovarian cancer cells. (C) 2000 Academic Press.