Effusion cytomorphology of small round cell tumors.
Effusion cytomorphology of small round cell tumors.
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DOI：
10.4103/0970-9371.182529
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发表时间：
2016-04
影响因子：
1.3
通讯作者：
Tsuta K
中科院分区：
文献类型：
--
作者：
Ikeda K;Tsuta K
小圆细胞肿瘤（SRCT）是一组由小，圆形和均匀的细胞组成的肿瘤，具有高的核/胞质（N/C）比。SRCT瘤细胞在渗出液中的出现是非常罕见的。我们报告了分泌液细胞学中SRCT的细胞形态学表现，并进行了统计学和数学分析，以区分SRCT。我们分析了40例SRCT积液标本的细胞学结果，并测量了细胞核长度、细胞间隙和细胞簇面积。SRCT包括尤文肉瘤（EWS）/原始神经外胚层肿瘤14例，滑膜肉瘤5例，横纹肌肉瘤6例，小细胞肺癌（SCLC）9例，弥漫性大B细胞淋巴瘤（DLBL）6例。在形态学上，在EWS、滑膜肉瘤和横纹肌肉瘤的病例中，细胞核和细胞质长度没有显著差异。SCLC和DLBL的胞浆长度小于EWS、滑膜肉瘤和横纹肌肉瘤。小细胞肺癌细胞核密度明显高于其他小细胞肺癌，DLBL细胞核不均匀，红细胞大小不等。我们相信，通过对渗出液样本的细胞学分析，诊断DLBL和SCLC是可能的，但很难用这种方法来区分EWS、滑膜肉瘤和横纹肌肉瘤。统计和数学分析表明，核密度和核和胞质大小的分散度是有用的参数，以传统的细胞学诊断标准，这是从经验中获得的。
Small round cell tumors (SRCTs) are a group of tumors composed of small, round, and uniform cells with high nuclear/cytoplasmic (N/C) ratios. The appearance of SRCT neoplastic cells in the effusion fluid is very rare. We reported the cytomorphological findings of SRCTs in effusion cytology, and performed statistical and mathematical analyses for a purpose to distinguish SRCTs. We analyzed the cytologic findings of effusion samples from 40 SRCT cases and measured the lengths of the nuclei, cytoplasms, and the cell cluster areas. The SRCT cases included 14 Ewing sarcoma (EWS)/primitive neuroectodermal tumor cases, 5 synovial sarcoma cases, 6 rhabdomyosarcoma cases, 9 small cell lung carcinoma (SCLC) cases, and 6 diffuse large B-cell lymphoma (DLBL) cases. Morphologically, there were no significant differences in the nuclear and cytoplasmic lengths in cases of EWS, synovial sarcoma, and rhabdomyosarcoma. The cytoplasmic lengths in cases of SCLC and DLBL were smaller than those of EWS, synovial sarcoma, and rhabdomyosarcoma. The nuclear density of the cluster in SCLC was higher than that in other SRCTs, and cases of DLBL showed a lack of anisokaryosis and anisocytosis. We believe that it might be possible to diagnose DLBL and SCLC from cytologic analysis of effusion samples but it is very difficult to use this method to distinguish EWS, synovial sarcoma, and rhabdomyosarcoma. Statistical and mathematical analyses indicated that nuclear density and dispersion of nuclear and cytoplasmic sizes are useful adjuncts to conventional cytologic diagnostic criteria, which are acquired from experience.