PAHs biodegradation potential of indigenous consortia from agricultural soil and contaminated soil in two-liquid-phase bioreactor (TLPB).
PAHs biodegradation potential of indigenous consortia from agricultural soil and contaminated soil in two-liquid-phase bioreactor (TLPB).
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DOI：
10.1016/j.jhazmat.2009.10.123
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发表时间：
2010-04
影响因子：
13.6
通讯作者：
Congying Wang;Fang Wang;Tao Wang;Y. Bian;Xing-lun Yang;Xin Jiang
中科院分区：
文献类型：
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作者：
Congying Wang;Fang Wang;Tao Wang;Y. Bian;Xing-lun Yang;Xin Jiang
评估土著菌群对多环芳烃的降解潜力是修复污染土壤的基础。采用双液相生物反应器（TLPB）对11种多环芳烃（PAHs）混合物进行了生物降解研究，以长期受PAHs污染的土壤（CS）和未受污染的农田土壤（AS）为接种物，比较了两种土壤中PAHs的生物降解效果。在TLPB中，硅油被用作有机相，以提高多环芳烃的生物利用度。在生物降解过程中，微生物数量也进行了测定。结果表明，萘、芴、菲、蒽、荧蒽和芘在两种土壤中均可在4-50天内完全生物降解。除二苯并（a，h）蒽外，苯并（a）蒽、苯并（a）芘、苯并（B）荧蒽和苯并（k）荧蒽在两种土壤中均有不同程度的降解。苯并（a）蒽和苯并（B）荧蒽的完全生物降解仅发生在CS中。在此过程中，微生物的生长与多环芳烃的生物降解密切相关。多环芳烃的顺序利用在多底物体系中表现出竞争抑制作用。在这里获得的多环芳烃的半衰期比以前报道的土壤中的短得多，这表明土著微生物在两种土壤中具有高的多环芳烃降解潜力，促进TLPB。
Estimation of PAHs degradation potential of indigenous consortia is essential for remediation of polluted soils. In this study, the biodegradation of a mixture of 11 PAHs was compared using a long-term PAH-contaminated soil (CS) and an unpolluted agricultural soil (AS) as inocula in a two-liquid-phase bioreactor (TLPB). In the TLPB, silicone oil was used as the organic phase to increase the PAHs bioavailability. The microbial numbers were also determined during the biodegradation. The results demonstrated that naphthalene, fluorene, phenanthrene, anthracene, fluoranthene and pyrene could be completely biodegraded in both soils within 4–50 days. With the exception of dibenzo(a,h)anthrancene, the other PAHs including benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene and benzo(k)fluoranthene were degraded to different extents in both soils at the end of 170 days. Complete biodegradation of benzo(a)anthracene and benzo(b)fluoranthene only occurred in CS. During the process, microbial growth was highly correlated to the biodegradation of PAHs. Sequential utilization of PAHs showed a competitive-inhibition in the multi-substrate system. The half-life times of PAHs obtained here were much shorter than those reported previously in soils, indicating that indigenous microbes in both soils had high PAHs degradation potential, facilitated by TLPB.