Ligament regeneration using a knitted silk scaffold combined with collagen matrix
Ligament regeneration using a knitted silk scaffold combined with collagen matrix
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使用针织丝支架结合胶原基质进行韧带再生
DOI：
10.1016/j.biomaterials.2008.05.017
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发表时间：
2008-09-01
期刊：
影响因子：
14
通讯作者：
Ouyang, Hong-Wei
中科院分区：
文献类型：
--
作者：
Chen, Xiao;Qi, Yi-Ying;Ouyang, Hong-Wei
本研究旨在通过蚕丝纤维、针织结构和胶原基质的协同作用，开发一种新型实用的韧带支架。在体外和动物模型中观察其对韧带组织工程的效果。培养在胶原基质上的细胞比在丝绸基质上培养的细胞表达更高水平的韧带基质基因。在小鼠模型中，丝素支架几乎没有引起炎症反应，皮下植入后降解缓慢。在兔MCL缺损模型中，丝素+胶原支架处理的MCL比未处理的MCL沉积了更多的胶原，具有更好的力学性能，表现出更自然的显微结构，胶原纤维直径更大，支架-韧带界面愈合更强。这些结果表明，丝绸+胶原海绵支架通过调节韧带基质基因表达和胶原纤维的组装，改善了韧带的结构和功能修复。这一发现首次强调了生物材料在韧带再生生物学中的重要作用。同时，提出了物理载荷作用下组织修复的“内部保留型”支架的概念。(C)2008爱思唯尔有限公司。保留所有权利。
This study was aimed to develop a new practical ligament scaffold by synergistic incorporation of silk fibers, a knitted structure, and a collagen matrix. The efficacy for ligament tissue engineering was investigated in vitro and in animal models. Cells cultured on a collagen substrate expressed ligament matrix genes at higher levels than those on a silk substrate. The silk scaffold elicited little inflammatory reaction and degraded slowly after subcutaneous implantation in a mouse model. In the rabbit MCL defect model, MCLs treated with a silk + collagen scaffold deposited more collagen, had better mechanical properties, and showed more native microstructure with larger diameter collagen fibrils and stronger scaffold-ligament interface healing than untreated MCLs and those treated with silk scaffolds. These results demonstrated that the knitted silk + collagen sponge scaffold improves Structural and functional ligament repair by regulating ligament matrix gene expression and collagen fibril assembly. The findings are the first to highlight the important roles of biomaterials in ligament regeneration biology. Also, the concept of an "internal-space-preservation" scaffold is proposed for the tissue repair under physical loading. (C) 2008 Elsevier Ltd. All rights reserved.