Survival of parvovirus B19-infected cells by cellular autophagy
Survival of parvovirus B19-infected cells by cellular autophagy
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DOI：
10.1016/j.virol.2006.03.029
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发表时间：
2006-06-05
期刊：
影响因子：
3.7
通讯作者：
Sugamura, Kazuo
中科院分区：
文献类型：
--
作者：
Nakashima, Akitoshi;Tanaka, Nobuyuki;Sugamura, Kazuo
人细小病毒B19 （B19）是一种众所周知的导致红细胞系细胞凋亡的病原体。在这里，我们提供了第一个证据，证明线粒体自噬在B19感染的细胞中特异性发现。感染细胞中LC3-II/LC3-I蛋白表达比显著升高，提示感染过程可能涉及细胞自噬。免疫荧光共聚焦显微镜分析显示，内源性LC3染色判断B19感染诱导细胞内自噬体。此外，3-MA对自噬的抑制显著促进了b19感染介导的细胞死亡。这些结果提示了b19感染细胞通过细胞自噬存活的新机制。(c) 2006爱思唯尔公司版权所有。
Human parvovirus B19 (B19) is a well-known pathogenic agent which causes apoptosis in erythrocyte lineage cells Here, we provide the first evidence that mitochondrial autophagy is specifically found in the B19-infected cells. The protein expression ratio for LC3-II/LC3-I increased significantly in infected cells, indicating possible involvement of cellular autophagy in the infection process. Immunofluorescence confocal microscopy analyses revealed that B19 infection induced an intracellular autophagosome as judged by endogenous LC3 staining. Moreover, inhibition of autophagy by 3-MA significantly facilitated B19-infection-mediated cell death. These results suggest a novel mechanism by which B19-infected cells survive by cellular autophagy. (c) 2006 Elsevier Inc. All rights reserved.