Expression of the cadherin-11 gene is a discriminative factor between articular and growth plate chondrocytes.
Expression of the cadherin-11 gene is a discriminative factor between articular and growth plate chondrocytes.
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DOI：
10.1016/j.joca.2005.10.008
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发表时间：
2006-04
影响因子：
7
通讯作者：
T. Matsusaki;T. Aoyama;K. Nishijo;T. Okamoto;T. Nakayama;T. Nakamura;J. Toguchida
中科院分区：
文献类型：
--
作者：
T. Matsusaki;T. Aoyama;K. Nishijo;T. Okamoto;T. Nakayama;T. Nakamura;J. Toguchida
肥大软骨细胞的钙化是生长板分化的最后一步，尽管确切的机制尚不清楚。我们已经从p53−/−小鼠中建立了两种生长板衍生的软骨细胞系，MMR 14和MMR 17（Nakamata T，Aoyama T，Okamoto T，Hoshijo T，Nishijo K，Nakayama T，et al. In vitro demonstration of cell-to-cell interaction in growth plate cartilage using chondrocytes established from p53−/− mice. J Bone Miner Res 2003;18：97-107）。长期体外培养在MMR 14中产生钙化结节，但在MMR 17中没有。我们利用cDNA微阵列技术比较了MMR 14和MMR 17的基因表达谱，以确定参与钙化过程的候选基因。结果发现，在MMR 14中有45个基因表达上调，其中包括钙粘蛋白11（Cdh-11）基因。Cdh-11在MMR 14中的表达在细胞-细胞连接处检测到，而在MMR 17中未观察到表达。原代培养的生长板软骨细胞也表达Cdh-11，Cdh-11主要分布于生长板晚期肥大区。细胞聚集试验表明，软骨细胞需要Ca 2+形成结节，并敲低Cdh-11基因的表达，使用短干扰RNA抑制钙化结节的形成MMR 14。引入Cdh-11到MMR 17未能产生钙化结节，这表明Cdh-11是一个，但不是唯一的，负责生产钙化nodules. CONCLUSION的因素，虽然生理作用还不清楚，Cdh-11是一个区别因素关节和生长板软骨细胞之间。
OBJECTIVECalcification of hypertrophic chondrocytes is the final step in the differentiation of growth plates, although the precise mechanism is not known. We have established two growth plate-derived chondrocyte cell lines, MMR14 and MMR17, from p53−/− mice (Nakamata T, Aoyama T, Okamoto T, Hosaka T, Nishijo K, Nakayama T, et al. In vitro demonstration of cell-to-cell interaction in growth plate cartilage using chondrocytes established from p53−/− mice. J Bone Miner Res 2003;18:97–107). Prolonged in vitro culture produced calcified nodules in MMR14, but not in MMR17. Factors responsible for the difference in calcification between the two cell lines may also be involved in the physiological calcification in growth plate.DESIGNGene expression profiles of MMR14 and MMR17 were compared using a cDNA microarray to identify candidate genes involved in the calcification process.RESULTSForty-five genes were identified as upregulated in MMR14, including the cadherin-11 (Cdh-11) gene. The expression of Cdh-11 in MMR14 was detected in cell–cell junctions, while no expression was observed in MMR17. Primary cultured chondrocytes from growth plate (GC) also expressed the Cdh-11, and the staining of Cdh-11 was observed in the late hypertrophic zone of growth plate. Cell aggregation assays showed that chondrocytes required Ca2+to form nodules, and knockdown of the Cdh-11 gene expression using short interfering RNA inhibited the formation of calcified nodules in MMR14. The introduction of Cdh-11 into MMR17 failed to produce calcified nodules indicating that Cdh-11 is one, but not the sole, factor responsible for the production of calcified nodules.CONCLUSIONAlthough the physiological role is still unclear, Cdh-11 is a discriminative factor between articular and growth plate chondrocytes.