Disruption of sperm release from insect testes by cytochalasin and β-actin mRNA mediated interference
Disruption of sperm release from insect testes by cytochalasin and β-actin mRNA mediated interference
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DOI：
10.1007/s00018-003-3139-z
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发表时间：
2003-08-01
影响因子：
8
通讯作者：
Giebultowicz, JM
中科院分区：
文献类型：
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作者：
Gvakharia, BO;Bebas, P;Giebultowicz, JM
蛾类精巢释放精子束受局部昼夜振荡器控制。限制精子束每天迁移几个小时的机制尚不清楚。我们证明，精子释放的每日周期是由折叠的无核精子束通过睾丸基部的细胞屏障的迁移启动的。这些纤维束在其近端有明显的肌动蛋白丝聚集。细胞松弛素在一天中特定时间抑制肌动蛋白聚合可抑制精子从睾丸中释放。同样，应用双链肌动蛋白RNA特异性抑制精子释放。这种RNA介导的干扰（RNAi）降低了参与精子释放的组织中肌动蛋白mRNA的水平。mRNA水平的下降导致无核精子束尖端的F-肌动蛋白的选择性耗竭，表明该肌动蛋白可能参与精子释放的启动。在生理、细胞和分子水平上的RNAi实验的综合结果确定了关键参与精子释放机制的独特细胞。
Release of sperm bundles from moth testes is controlled by the local circadian oscillator. The mechanism which restricts migration of sperm bundles to a few hours each day is not understood. We demonstrate that a daily cycle of sperm release is initiated by the migration of folded apyrene sperm bundles through a cellular barrier at the testis base. These bundles have conspicuous concentrations of actin filaments at their proximal end. Inhibition of actin polymerization by cytochalasin at a specific time of day inhibited sperm release from the testis. Likewise, application of double-stranded actin RNA specifically inhibited sperm release. This RNA-mediated interference (RNAi) lowered the pool of actin mRNA in tissues involved in sperm release. The decline in mRNA levels resulted in the selective depletion of F-actin from the tip of apyrene sperm bundles, suggesting that this actin may be involved in the initiation of sperm release. Combined results of RNAi experiments at physiological, cellular and molecular levels identified unique cells that are critically involved in the mechanism of sperm release.