Phylogeny of Wolbachia in filarial nematodes
Phylogeny of Wolbachia in filarial nematodes
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DOI：
10.1098/rspb.1998.0591
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发表时间：
1998-12-22
影响因子：
4.7
通讯作者：
Blaxter, ML
中科院分区：
文献类型：
--
作者：
Bandi, C;Anderson, TJC;Blaxter, ML
在不同种类的丝虫线虫(丝虫科)中观察到了胞内细菌。犬丝虫的胞内细菌已被证明与沃尔巴克氏菌密切相关，沃尔巴克氏菌是一种类似立克次体的微生物，广泛存在于节肢动物中。然而，不同丝虫内共生菌之间的关系，以及它们与节肢动物沃尔巴克氏菌之间的关系似乎还没有被研究过。为了解决这些问题，我们检查了十种丝虫线虫中沃尔巴克氏菌的存在。对于9个物种，使用针对沃巴赫菌FtsZ基因的特异性引物进行检测，所有样品都是阳性的。对于一个物种，检查的样本是PCR阴性的。丝虫沃尔巴克氏菌FtsZ基因片段的序列分为两个簇(C和D)，这两个簇与已知的节肢动物沃尔巴克氏菌的A和B簇不同。这四个谱系(A-D)在一个星状的系统发育中是相关的，C和D之间的核苷酸差异大于A和B之间的核苷酸差异。此外，在丝虫沃尔巴克氏菌的两个谱系中，共生菌的系统发育与宿主的系统发育是一致的。因此，没有证据表明最近沃尔巴克氏菌在节肢动物和线虫之间传播。丝虫物种子集的内共生体16S核糖体DNA序列支持这些发现。
Intracellular bacteria have been observed in various species of filarial nematodes (family Onchocercidae). The intracellular bacterium of the canine filaria Dirofilaria immitis has been shown to be closely related to Wolbachia, a rickettsia-like micro-organism that is widespread among arthropods. However, the relationships between endosymbionts of different filariae, and between these and the arthropod wolbachiae, appear not to have been studied. To address these issues we have examined ten species of filarial nematodes for the presence of Wolbachia. For nine species, all samples examined were PCR positive using primers specific for the ftsZ gene of Wobbachia. For one species, the examined samples were PCR negative. Sequences of the amplified ftsZ gene fragments of filarial wolbachiae fall into two clusters (C and D), which are distinct from the A and B clusters recognized for arthropod wolbachiae. These four lineages (A-D) are related in a star-like phylogeny, with higher nucleotide divergence observed between C and D wolbachiae than that observed between A and B wolbachiae. In addition, within each of the two lineages of filarial wolbachiae, the phylogeny of the symbionts is consistent with the host phylogeny. Thus, there is no evidence for recent Wolbachia transmission between arthropods and nematodes. Endosymbiont 16S ribosomal DNA sequences from a subset of filarial species support these findings.