Both early and late Drosophila chorion gene promoters confer correct temporal, tissue and sex specificity on a reporter Adh gene.
Both early and late Drosophila chorion gene promoters confer correct temporal, tissue and sex specificity on a reporter Adh gene.
复制标题
早期和晚期果蝇绒毛膜基因启动子都赋予报告基因 Adh 正确的时间、组织和性别特异性。
作者：
C. Romano;B. Bienz;B. Mariani;F. Kafatos
In vivo transformation studies have been performed using fusion constructs of chorion DNA and the alcohol dehydrogenase (Adh) structural gene. The results indicate that almost exclusively 5′ flanking DNA regions of the early (s36) and late (s15) chorion genes suffice for conferring normal chorion developmental specificity (sex, tissue and temporal) on the reporter gene. In the case of s15, the proximal 5′ flanking DNA up to position −370 is sufficient for specificity. However, quantitative analysis indicates that one or more elements within or downstream of the s15 gene are required, either transcriptionally or post‐transcriptionally, for attainment of an mRNA level comparable to that of the endogenous s15 gene (corrected for amplification); in the absence of such element(s), the average level of Adh transcript produced by fusion gene constructs is 18‐fold lower.
