A decrease in phytic acid content substantially affects the distributionof mineral elements within rice seeds
A decrease in phytic acid content substantially affects the distributionof mineral elements within rice seeds
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植酸含量的降低显着影响水稻种子内矿物质元素的分布
DOI：
10.1016/j.plantsci.2015.06.006
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发表时间：
2015
期刊：
影响因子：
5.2
通讯作者：
Kaoru T. Yoshida
中科院分区：
文献类型：
--
作者：
Hiroaki Sakai;Toru Iwai;Chie Matsubara;Yuto Usui;Masaki Okamura,Osamu Yatou;Yasuko Terada;Naohiro Aoki;Sho Nishida;Kaoru T. Yoshida
植酸（肌醇六磷酸; InsP 6）是种子中磷和许多矿物元素的储存化合物。为了确定InsP 6在种子中矿物质元素积累和分布中的作用，我们使用来自两个独立的水稻低植酸（lpa）突变体的发育种子，通过基于同步加速器的X射线显微荧光分析对矿物质元素进行了精细绘图。（Oryza sativaL.）。降低InsP 6酶活性并不影响矿质元素从营养器官向种子的转运，因为酶活性降低的种子中磷和其它矿质元素的总量与野生型（WT）相同。然而，减少InsP 6引起的大的变化，矿物定位在lpases。糊粉层中的磷、钾含量显著降低，并向胚乳中扩散。锌和铜在野生型中广泛分布于糊粉层至胚乳内部，而在突变体中则分布于糊粉层周围较窄的空间。我们还证实了类似的分布变化发生在转基因水稻与lpa表型。利用这些结果，我们讨论了InsP 6在种子发育过程中矿质元素的动态积累和分配格局中的作用。
Phytic acid (myo-inositol hexakisphosphate; InsP6) is the storage compound of phosphorus and many mineral elements in seeds. To determine the role of InsP6in the accumulation and distribution of mineral elements in seeds, we performed fine mappings of mineral elements through synchrotron-based X-ray microfluorescence analysis using developing seeds from two independent low phytic acid (lpa) mutants of rice (Oryza sativaL.). The reduced InsP6inlpaseeds did not affect the translocation of mineral elements from vegetative organs into seeds, because the total amounts of phosphorus and the other mineral elements inlpaseeds were identical to those in the wild type (WT). However, the reduced InsP6caused large changes in mineral localization withinlpaseeds. Phosphorus and potassium in the aleurone layer oflpagreatly decreased and diffused into the endosperm. Zinc and copper, which were broadly distributed from the aleurone layer to the inner endosperm in the WT, were localized in the narrower space around the aleurone layer inlpamutants. We also confirmed that similar distribution changes occurred in transgenic rice with the lpa phenotype. Using these results, we discussed the role of InsP6in the dynamic accumulation and distribution patterns of mineral elements during seed development.