Circulating miR-143 and miR-145 as promising biomarkers for evaluating severity of coronary artery stenosis in patients with acute coronary syndrome
Circulating miR-143 and miR-145 as promising biomarkers for evaluating severity of coronary artery stenosis in patients with acute coronary syndrome
复制标题
循环 miR-143 和 miR-145 作为评估急性冠状动脉综合征患者冠状动脉狭窄严重程度的有希望的生物标志物
DOI：
10.1016/j.clinbiochem.2022.10.004
复制
发表时间：
2023
影响因子：
2.8
通讯作者：
Hui Zhu
中科院分区：
文献类型：
--
作者：
Linlin Meng;Xin Yu;Haitao Han;Xu Jia;Boang Hu;Lei Zhang;Zhihao Wang;Wei Zhang;Ming Zhong;Hui Zhu
背景多项研究表明miR-143和miR-145在动脉粥样硬化的发生发展中起重要作用。本研究旨在探讨急性冠脉综合征(ACS)患者血浆miR-143和miR-145的表达及其与冠状动脉狭窄程度的关系。方法279例急性冠脉综合征患者包括201例不稳定型心绞痛(UA)和78例急性心肌梗死(AMI)患者，65例正常对照组。采用实时定量聚合酶链式反应(qRT-PCR)检测血浆miR-143和miR-145水平。结果急性冠脉综合征患者血浆miR-143、miR-145水平均低于对照组(P<0.001)。血浆MIR-143和MIR-145水平与Gensini评分呈负相关(MIR-143：R=−0.246，P<；0.001；MIR-145：R=−0.222，P<；0.001)。Logistic回归分析显示，miR-143和miR-145分别是急性冠脉综合征、不稳定型心绞痛和急性心肌梗死发病的保护因素。MIR-143(AUC：0.786，p<；0.001)和MIR-145(AUC：0.793，P<；(0.001)可预测冠状动脉狭窄程度>50%。结论急性冠脉综合征患者血浆MIR-143和MIR-145水平显著降低，且与冠脉狭窄程度呈负相关。结论：血浆miR-143和miR-145水平可作为评价冠状动脉狭窄程度的无创性生物标志物。
BackgroundMultiple studies have provided evidence that miR-143 and miR-145 play a crucial role in the pathogenesis and progression of atherosclerosis. In the present study, we aim to investigate the expression of plasma miR-143 and miR-145 in patients with acute coronary syndrome (ACS) and their association with the severity of coronary artery stenosis.MethodsThe study enrolled 279 patients with ACS, including 201 patients with unstable angina (UA) and 78 patients with acute myocardial infarction (AMI), and 65 matched subjects as the control group. The plasma levels of miR-143 and miR-145 were detected by quantitative real-time PCR (qRT-PCR). Gensini score was applied to evaluate the severity of coronary artery stenosis.ResultsPlasma levels of miR-143 and miR-145 in patients with ACS were both decreased compared with the control group (p< 0.001). Plasma levels of miR-143 and miR-145 were negatively correlated with Gensini score (miR-143: r = −0.246,p< 0.001; miR-145: r = −0.222,p< 0.001). Logistic regression analysis showed that miR-143 and miR-145 were protective factors for the onset of ACS, UA, or AMI separately. MiR-143 (AUC: 0.786,p< 0.001) and miR-145 (AUC: 0.793,p< 0.001) were able to predict the degree of coronary artery stenosis greater than 50 %.ConclusionThe plasma levels of miR-143 and miR-145 were significantly decreased in ACS patients and were negatively correlated with coronary stenosis. In conclusion, plasma miR-143 and miR-145 levels can be used as noninvasive biomarkers for evaluating coronary artery stenosis.