ACCUMULATION OF A NOVEL GLYCOLIPID AND A BETAINE LIPID IN CELLS OF RHODOBACTER-SPHAEROIDES GROWN UNDER PHOSPHATE LIMITATION
ACCUMULATION OF A NOVEL GLYCOLIPID AND A BETAINE LIPID IN CELLS OF RHODOBACTER-SPHAEROIDES GROWN UNDER PHOSPHATE LIMITATION
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DOI：
10.1006/abbi.1995.1141
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发表时间：
1995-02-20
影响因子：
3.9
通讯作者：
GAGE, DA
中科院分区：
文献类型：
--
作者：
BENNING, C;HUANG, ZH;GAGE, DA
光合细菌Rhodobacter sphaeroides的细胞在磷酸盐限制条件下生长，积累了非磷糖脂和携带来自氨基酸的头部基团的脂质。同时，磷酸甘油脂的相对量从膜中总极性脂质的90摩尔%下降到22摩尔%。两种脂质，在标准条件下生长的细胞中检测不到，在磷酸盐限制的生长过程中合成。快速原子轰击质谱法、精确质量测量、H-1 NMR光谱法、糖组成分析和主要糖脂的甲基化分析鉴定了新型化合物1，2-二-O-酰基-3-O-[α-D-吡喃葡萄糖基-（1 -> 4）-O-β-D-吡喃半乳糖基]甘油。通过采用来自莱茵衣藻的真实标准品的共色谱法、快原子轰击质谱法、精确质量测量法和1H NMR光谱法，将第二种脂质鉴定为甜菜碱脂质1，2-二-O-酰基-[4 '-（N，N，N-三甲基）高丝氨酸]甘油。在此观察之前，这种脂质的出现被认为仅限于低等植物和藻类。显然，这些新合成的非磷脂质，除了在R. sphaeroides在最佳条件下生长，在磷酸盐剥夺的细胞中接管磷酸甘油脂的作用。(C)出版社：Academic Press
Cells of the photosynthetic bacterium Rhodobacter sphaeroides grown under phosphate-limiting conditions accumulated nonphosphorous glycolipids and lipids carrying head groups derived from amino acids. Concomitantly, the relative amount of phosphoglycerolipids decreased from 90 to 22 mol% of total polar lipids in the membranes. Two lipids, not detectable in cells grown under standard conditions, were synthesized during phosphate-limited growth. Fast atom bombardment mass spectroscopy, exact mass measurements, H-1 NMR spectroscopy, sugar composition analysis, and methylation analysis of the predominant glycolipid led to the identification of the novel compound 1,2-di-O-acyl-3-O-[alpha-D-glucopyranosyl-(1 -> 4)-O-beta-D-galactopyranosyl]glycerol. The second lipid was identified as the betaine lipid 1,2-di-O-acyl-[4'-(N,N,N-trimethyl)homoserine]glycerol by cochromatography employing an authentic standard from Chlamydomonas reinhardtii, fast atom bombardment mass spectroscopy, exact mass measurements, and 1H NMR spectroscopy. Prior to this observation, the occurrence of this lipid was thought to be restricted to lower plants and algae. Apparently, these newly synthesized nonphosphorous lipids, in addition to the sulfo- and the ornithine lipid also found in R. sphaeroides grown under optimal conditions, take over the role of phosphoglycerolipids in phosphate-deprived cells. (C) 1995 Academic Press, Inc.