Alicyclobacillus aeris sp nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine
Alicyclobacillus aeris sp nov., a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine
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Alicyclobacillus aeris sp nov.，一种从铜矿中分离出来的新型亚铁和硫氧化细菌
DOI：
10.1099/ijs.0.008870-0
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发表时间：
2009-10-01
影响因子：
2.8
通讯作者：
Jiang, Cheng-Ying
中科院分区：
文献类型：
--
作者：
Guo, Xu;You, Xiao-Yan;Jiang, Cheng-Ying
从内蒙古紫金铜矿中分离到一株新的嗜中温、嗜酸性内生孢子形成菌，命名为ZJ-6（T）。菌株ZJ-6（T）的细胞呈杆状，染色为革兰氏阳性或革兰氏可变，并在25-35 ℃（最适，30 ℃）和pH 2.0-6.0（最适，pH 3.5）下有氧生长。16 S rRNA基因序列分析表明，菌株ZJ-6（T）与脂环酸芽孢杆菌属（Alicyclobacillus）的成员具有亲缘关系，其16 S rRNA基因序列相似性为89.5- 94.2%。细胞含有MK-7作为主要醌，DNA G + C含量为51.2mol%。菌株ZJ-6（T）具有许多表型特征，包括其生长温度，各种碳源的同化，从一系列化合物产生酸，以及使用亚铁、元素硫和连四硫酸盐作为电子供体进行化学自养生长的能力，这些表型特征使其与公认的脂环酸芽孢杆菌物种区分开。菌株ZJ-6（T）的主要脂肪酸为antiso-C-15：0（67.1%），异-C-16：0（7.7%）和antiso-C-17：0（7.4%）;未发现ω-脂环族脂肪酸。根据这些结果，得出结论：菌株ZJ-6（T）代表脂环酸杆菌属中的一个新物种，建议将其命名为脂环酸杆菌（Alicyclobacillusaeris sp. nov.）;模式菌株为ZJ-6（T）（=CGMCC 1.7072（T）=NBRC 104953（T））。
A novel mesophilic, acidophilic, endospore-forming bacterium, designated strain ZJ-6(T), was isolated from Zi-Jin copper mine in Inner Mongolia, China. Cells of strain ZJ-6(T) were rod-shaped, stained Gram-positive or were Gram-variable, and grew aerobically at 25-35 degrees C (optimum, 30 degrees C) and pH 2.0-6.0 (optimum, pH 3.5). 16S rRNA gene sequence analysis showed that strain ZJ-6(T) was related phylogenetically to members of the genus Alicyclobacillus, with 16S rRNA gene sequence similarities of 89.5-94.2 %. Cells contained MK-7 as the major quinone and the DNA G + C content was 51.2 mol%. Strain ZJ-6(T) possessed a number of phenotypic characteristics that differentiated it from recognized Alicyclobacillus species, including its growth temperature, assimilation of various carbon sources, production of acids from a range of compounds, and the ability to grow chemoautotrophically using ferrous iron, elemental sulfur and tetrathionate as electron donors. The predominant cellular fatty acids of strain ZJ-6(T) were anteiso-C-15:0 (67.1 %), iso-C-16:0 (7.7 %) and anteiso-C-17:0 (7.4 %); omega-alicyclic fatty acids were not found. On the basis of these results, it is concluded that strain ZJ-6(T) represents a novel species within the genus Alicyclobacillus, for which the name Alicyclobacillus aeris sp. nov. is proposed; the type strain is ZJ-6(T) (=CGMCC 1.7072(T) =NBRC 104953(T)).