Digital Light 4D Printing of Bioresorbable Shape Memory Elastomers for Personalized Biomedical Implantation.
Digital Light 4D Printing of Bioresorbable Shape Memory Elastomers for Personalized Biomedical Implantation.
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DOI：
10.1016/j.actbio.2024.02.009
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发表时间：
2024-02
影响因子：
9.7
通讯作者：
Alireza Mahjoubnia;Dunpeng Cai;Yuchao Wu;Skylar D. King;Pooya Torkian;Andy C. Chen;R. Talaie;Shi-You Chen;Jian Lin
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文献类型：
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作者：
Alireza Mahjoubnia;Dunpeng Cai;Yuchao Wu;Skylar D. King;Pooya Torkian;Andy C. Chen;R. Talaie;Shi-You Chen;Jian Lin
四维(4D)打印为个性化生物医学植入打开了新的潜力，但仍然存在缺乏合适材料的障碍。在这里，我们展示了一种生物可吸收形状记忆弹性体(SME)，在其相变温度(TTRANS)以下和之上都具有高弹性。这种SME可以通过与丙烯酸单体共聚十二酸甘油丙烯酸酯预聚体(Pre-PGDA)形成交联型聚(十二酸甘油丙烯酸酯)(PGDA)-聚丙烯酸(PAA)或PGDA-PAA网络来进行数字光3D打印。打印的复杂、独立的3D结构具有高分辨率特征，在生理温度下显示出形状编程特性。通过在55wt%和70wt%之间调节Pre-PGDA重量比，T在39.2wt%和47.2MPa.℃之间变化，杨氏模数(E)在40-170Mpa范围内变化，断裂应变(εf)在170%-200%之间变化。反之，E降至1-1.82 Mpa，与软组织接近。值得注意的是，ε仍保持在130-180%。在材料的体外生物相容性测试中，90%的细胞增殖和良好的细胞附着。在活体血管移植试验中，强调了这些4D打印结构在再生主动脉组织方面的几何和机械适应性。植入物的生物降解表明，随着时间的推移，它们有可能被自然组织完全取代。为了突出其个性化医疗的潜力，打印了患者专用的左心耳(LAA)封堵器，并将其植入体外心脏模型中。意义陈述专门为患者设计和制造的4D打印形状记忆弹性体(SME)植入物在微创手术(MIS)中广受欢迎。这些植入物中使用的传统形状记忆聚合物通常存在转变温度不合适、生物兼容性差、3D设计复杂性有限和韧性低等问题，使其不适合用于管理信息系统。我们的新型SME具有可调节的转变温度和增强的韧性，具有生物兼容性和自然可降解性，特别是在心血管环境中。这使得植入物，如生物医学支架，可以在室温下进行编程，然后适应植入后身体的生理条件。我们的研究，包括活体血管移植和体外装置植入，突出了SME在主动脉组织再生中的有效性及其在管理信息系统中的应用前景。
Four-dimensional (4D) printing unlocks new potentials for personalized biomedical implantation, but still with hurdles of lacking suitable materials. Herein, we demonstrate a bioresorbable shape memory elastomer (SME) with high elasticity at both below and above its phase transition temperature (Ttrans). This SME can be digital light 3D printed by co-polymerizing glycerol dodecanoate acrylate prepolymer (pre-PGDA) with acrylic acid monomer to form crosslinked Poly(glycerol dodecanoate acrylate) (PGDA)-Polyacrylic acid (PAA), or PGDA-PAA network. The printed complex, free-standing 3D structures with high-resolution features exhibit shape programming properties at a physiological temperature. By tuning the pre-PGDA weight ratios between 55 wt% and 70 wt%,Ttransvaries between 39.2 and 47.2 ℃ while Young's moduli (E) range 40–170 MPa belowTtranswith fractural strain (εf) of 170 %-200 %. AboveTtrans, Edrops to 1–1.82 MPa which is close to those of soft tissue. Strikingly,εfof 130–180 % is still maintained.In vitrobiocompatibility test on the material shows > 90 % cell proliferation and great cell attachment.In vivovascular grafting trials underline the geometrical and mechanical adaptability of these 4D printed constructs in regenerating the aorta tissue. Biodegradation of the implants shows the possibility of their full replacement by natural tissue over time. To highlight its potential for personalized medicine, a patient-specific left atrial appendage (LAA) occluder was printed and implanted endovascularly into anin vitroheart model.Statement of significance4D printed shape-memory elastomer (SME) implants particularly designed and manufactured for a patient are greatly sought-after in minimally invasive surgery (MIS). Traditional shape-memory polymers used in these implants often suffer from issues like unsuitable transition temperatures, poor biocompatibility, limited 3D design complexity, and low toughness, making them unsuitable for MIS. Our new SME, with an adjustable transition temperature and enhanced toughness, is both biocompatible and naturally degradable, particularly in cardiovascular contexts. This allows implants, like biomedical scaffolds, to be programmed at room temperature and then adapt to the body's physiological conditions post-implantation. Our studies, includingin vivovascular grafts andin vitrodevice implantation, highlight the SME's effectiveness in aortic tissue regeneration and its promising applications in MIS.