A novel UV-B priming system reveals an UVR8-depedent memory, which provides resistance against UV-B stress in Arabldopsis leaves
A novel UV-B priming system reveals an UVR8-depedent memory, which provides resistance against UV-B stress in Arabldopsis leaves
复制标题
一种新型的 UV-B 启动系统揭示了 UVR8 依赖性记忆，可在拟南芥叶片中抵抗 UV-B 胁迫
DOI：
10.1080/15592324.2021.1879533
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发表时间：
2021-02-27
影响因子：
2.9
通讯作者：
Muller-Xing, Ralf
中科院分区：
文献类型：
--
作者：
Xiong, Ying;Xing, Qian;Muller-Xing, Ralf
用丁香假单胞菌等病原体对植物进行单一处理，可引发拟南芥持续数天至数周的系统获得性抗性（SAR）。对于干旱和热应激等非生物胁迫也描述了类似的引发抗性。大多数关于植物抵抗紫外线 (UV) 辐射的研究都长期使用低 UV-B 辐射。这些实验设计使得很难将适应效应与真实细胞记忆区分开来，真实细胞记忆促进潜伏期后对第二次紫外线辐射的转录和其他反应。在这里，我们展示了一种新型 UV-B 底漆系统。我们证明，单一 UV-B 处理既不会造成可见损伤，也不会引起色素积累，但可以增强对 UV-B 应激的抵抗力。经过第二次破坏性 UV-B 处理后，与未涂底漆的植物相比，涂过 UV-B 的植物受到的损害显着减少。此外，uvr8-6突变体中诱导的UV-B抗性的获得受到损害，这表明UV-B受体对于拟南芥中的UV-B应激记忆至关重要。我们讨论了 UV-B 底漆系统的优点和局限性，该系统将成为未来研究中研究 UV-B 记忆的强大工具。
Single treatment of plants with pathogens like Pseudomonas syringae can trigger systemic acquired resistance (SAR) that lasts several days to several weeks in Arabidopsis thaliana. Similar primed resistances were described for abiotic stresses like drought and heat stress. Most studies about plant resistance to ultraviolet (UV)-radiation used low UV-B radiations over a long period. These experimental designs make it difficult to distinguish acclimation effects from real cellular memory which facilitate transcriptional and other responses to a second UV-radiation after a latent phase. Here we present a novel UV-B priming system. We demonstrate that a single UV-B treatment, which causes neither visible damage nor accumulation of pigments, can stimulate resistance against UV-B stress. After a second damaging UV-B treatment, UV-primed plants showed significantly reduced damage in comparison to non-primed plants. Furthermore, the acquirement of the induced UV-B resistance was impaired in uvr8-6 mutants suggesting that the UV-B receptor is essential for UV-B stress memory in Arabidopsis. We discuss advantages and limits of our UV-B priming system which will be a powerful tool to investigate UV-B memory in future studies.