Mechanical feedback promotes bacterial adaptation to antibiotics
Mechanical feedback promotes bacterial adaptation to antibiotics
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DOI：
10.1038/s41567-020-01079-x
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发表时间：
2021-01-04
期刊：
影响因子：
19.6
通讯作者：
Dinner, Aaron R.
中科院分区：
文献类型：
--
作者：
Banerjee, Shiladitya;Lo, Klevin;Dinner, Aaron R.
为了最大限度地提高它们的适应性，细胞必须能够有效地应对压力。这就要求在保护资源以促进生存的过程和利用资源促进生长和分裂的过程之间进行权衡。理解这些权衡的性质及其背后的物理学仍然是一个突出的挑战。在这里，我们结合联合收割机单细胞实验和理论建模，提出了一种机制，通过细胞生长和形态之间的机械反馈抗生素适应。在长期暴露于亚致死剂量的核糖体靶向抗生素下，我们发现新月柄杆菌细胞可以恢复刺激前的生长速率，并发生细胞形状的巨大变化。在抗生素去除后，细胞在多代中恢复其原始形式。这些现象可以用细菌生长的物理理论来解释，该理论表明，细胞宽度和曲率的增加会在蛋白质合成抑制下促进更快的生长。形状的变化使细菌更能适应存活的抗生素。
To maximize their fitness, cells must be able to respond effectively to stresses. This demands making trade-offs between processes that conserve resources to promote survival, and processes that use resources to promote growth and division. Understanding the nature of these trade-offs and the physics underlying them remains an outstanding challenge. Here we combine single-cell experiments and theoretical modelling to propose a mechanism for antibiotic adaptation through mechanical feedback between cell growth and morphology. Under long-term exposure to sublethal doses of ribosome-targeting antibiotics, we find that Caulobacter crescentus cells can recover their pre-stimulus growth rates and undergo dramatic changes in cell shape. Upon antibiotic removal, cells recover their original forms over multiple generations. These phenomena are explained by a physical theory of bacterial growth, which demonstrates that an increase in cell width and curvature promotes faster growth under protein synthesis inhibition. Shape changes thereby make bacteria more adaptive to surviving antibiotics.