A novel approach for mechanical tissue characterization indicates decreased elastic strength in brain areas affected by experimental thromboembolic stroke.
A novel approach for mechanical tissue characterization indicates decreased elastic strength in brain areas affected by experimental thromboembolic stroke.
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DOI：
10.1097/wnr.0000000000000395
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发表时间：
2015-07-08
期刊：
影响因子：
1.7
通讯作者：
Fuhs T
中科院分区：
文献类型：
--
作者：
Michalski D;Härtig W;Krueger M;Hobohm C;Käs JA;Fuhs T
由于缺血性卒中的治疗仍然是一个挑战，相对于许多神经保护尝试的失败，有一个持续的需要，以更好地了解病理生理机制，造成组织损伤。虽然缺血性的结果已被广泛研究，在细胞和分子水平上使用组织学和生物化学方法，受缺血影响的脑组织的机械完整性方面的属性还没有得到解决。作为一种新的方法，本研究使用基于荧光的检测受实验性血栓栓塞性中风影响的区域，结合扫描力显微镜检查选定的大鼠脑区的机械变化。缺血发作后25小时，与对侧未受影响的半球相比，发现主要受缺血影响的纹状体的弹性强度降低。额外的半球内分析显示，与受影响更严重的纹状体相比，缺血边缘区的弹性强度降低。总之，这些数据有力地表明了局灶性脑缺血引起的机械组织完整性的关键改变。此外，根据已经获得的与缺血边缘区相关的数据，发现了与半影概念良好一致的壳状机械组织损伤模式。这些发现可能有助于开发特定的治疗干预措施，以保护受影响的区域免受机械完整性的严重损失。
As treatment of ischemic stroke remains a challenge with respect to the failure of numerous neuroprotective attempts, there is an ongoing need for better understanding of pathophysiological mechanisms causing tissue damage. Although ischemic outcomes have been studied extensively at the cellular and molecular level using histological and biochemical methods, properties of ischemia-affected brain tissue with respect to mechanical integrity have not been addressed so far. As a novel approach, this study used fluorescence-based detection of regions affected by experimental thromboembolic stroke in combination with scanning force microscopy to examine mechanical alterations in selected rat brain areas. Twenty-five hours after onset of ischemia, a decreased elastic strength in the striatum as the region primarily affected by ischemia was found compared with the contralateral nonaffected hemisphere. Additional intrahemispheric analyses showed decreased elastic strength in the ischemic border zone compared with the more severely affected striatum. In conclusion, these data strongly indicate a critical alteration in mechanical tissue integrity caused by focal cerebral ischemia. Further, on the basis of data that have been obtained in relation to the ischemic border zone, a shell-like pattern of mechanical tissue damage was found in good accordance with the penumbra concept. These findings might enable the development of specific therapeutic interventions to protect affected areas from critical loss of mechanical integrity.