Expression of a retinoic acid receptor gene in myeloid leukemia cells.
Expression of a retinoic acid receptor gene in myeloid leukemia cells.
复制标题
髓系白血病细胞中视黄酸受体基因的表达。
作者：
C. Wang;Curtis Je;Minden;E. McCulloch
视黄酸 (RA) 已被证明可以增加某些白血病细胞系（HL-60 和 KG-1）的分化，但不能增加其他白血病细胞系（K562）的分化。同样，据报道，RA 对新鲜胚细胞具有不同的影响。最近，已经获得了视黄酸核受体的分子克隆。本文描述的实验旨在比较髓母细胞白血病细胞中受体的表达与生物活性。在四种连续的 AML 细胞系中，通过 Northern 分析或 RNA 斑点印迹发现视黄酸受体 (RAR) 表达与 RA 抑制集落形成的能力呈正相关。对最敏感细胞系的动力学研究表明，集落形成的抑制与母细胞自我更新和分化样事件的减少有关。在 23 名 AML 患者新鲜获得的母细胞中检测到了 RAR。观察到患者之间的差异；然而，未发现 RAR 表达与新获得的母细胞对 RA 的反应之间存在相关性。
Retinoic acid (RA) has been shown to increase differentiation in some leukemia cell lines (HL-60 and KG-1) but not others (K562). Similarly, RA has been reported to have variable effects on fresh blast cells. Recently, molecular clones have been obtained for the nuclear receptor for retinoic acid. The experiments described in this paper were designed to compare expression of the receptor to biological activity in myeloblastic leukemia cells. In four continuous AML cell lines, a positive correlation was found between retinoic acid receptor (RAR) expression by Northern analysis or RNA dot blot and the ability of RA to inhibit colony formation. Kinetic studies of the most sensitive cell line showed that inhibition of colony formation was associated with reduced blast cell self-renewal and differentiation-like events. RAR was detected in freshly obtained blast cells from 23 AML patients. Patient-to-patient variation was observed; however, a correlation was not found between RAR expression and response of the freshly obtained blast cells to RA.