Differential in vivo cytokine mRNA expression in lesional skin of intrinsic vs. extrinsic atopic dermatitis patients using semiquantitative RT-PCR
Differential in vivo cytokine mRNA expression in lesional skin of intrinsic vs. extrinsic atopic dermatitis patients using semiquantitative RT-PCR
复制标题
DOI：
10.1111/j.1365-2222.2003.01782.x
复制
发表时间：
2003-12-01
影响因子：
6.1
通讯作者：
Yang, JM
中科院分区：
文献类型：
--
作者：
Jeong, CW;Ahn, KS;Yang, JM
背景一小群血清IgE水平正常且无特异性IgE致敏的特应性皮炎（AD）患者被称为“内在型AD”（ADi），作为术语“外在型AD”（ADe）的对应物。然而，在这方面，目的探讨ADi和ADe的发病机制及体内细胞因子微环境的变化。方法采用半定量RT-PCR方法，检测ADi和ADe的细胞因子微环境，并与对照组进行比较。PCR检测两种类型AD患者皮肤活检组织中细胞因子的表达，并评估组织嗜酸性粒细胞计数。ADe与ADi患者皮损中细胞浸润无显著性差异，组织嗜酸性粒细胞增多明显高于ADi患者。基于我们的RT-PCR，细胞因子的表达模式可以分为四组。第一组包括IL-5、IL-13和IL-1 β，其mRNA表达水平在两种类型的AD患者中均高于非特应性（NA）受试者，而ADe患者的水平甚至高于ADi患者。第二组包括干扰素-γ（IFN-γ）、IL-12、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、IL-4和IL-10，其mRNA表达水平在两种类型的AD患者中均升高，而在ADe和ADi患者之间无差异。第三类包括肿瘤坏死因子-α第四组包括IL-6和转化生长因子-β，结论IL-5、IL-13、TGF-β、TGF-β的表达在慢性胰腺炎患者中无明显差异，AD e患者皮肤IL-1 β mRNA表达及皮肤浸润嗜酸性粒细胞数均高于AD i患者。
Background A small subgroup of atopic dermatitis (AD) patients with normal serum IgE levels and without specific IgE sensitization has been termed 'intrinsic type of AD' (ADi) as a counterpart to the term 'extrinsic type of AD' (ADe). However, there are neither molecular markers nor clinically diagnostic tools for distinguishing between ADi and ADe.Objective The present studies were undertaken to clarify the pathogenesis and in vivo cytokine micromilieu of ADi patients in comparison with ADe patients.Methods We used semiquantitative RT-PCR to investigate the expression of various cytokines and assessed the tissue eosinophil counts in skin biopsies from both types of AD patients.Results Although there was no significant difference of cellular infiltrates in the lesional skin between ADe and ADi patients, ADe had significantly increased tissue eosinophilia than ADi. Based on our RT-PCR, the expression patterns of cytokines could be categorized into four groups. The first group includes IL-5, IL-13, and IL-1beta, whose levels of mRNA expression were higher in both types of AD patients than non-atopic (NA) subjects, while ADe patients had even higher levels than ADi patients. The second group includes interferon-gamma (IFN-gamma), IL-12, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-4, and IL-10, whose levels of mRNA expression were elevated in both types of AD patients without differences between ADe and ADi patients. The third group includes tumour necrosis factor-alpha (TNF-alpha), whose mRNA expression was more decreased in both types of AD patients than NA, and the fourth group includes IL-6 and transforming growth factor-beta (TGF-beta), which did not show any differences among the three groups.Conclusion These current data demonstrate that the expressions of cytokines IL-5, IL-13, and IL-1beta mRNA and the number of dermal infiltrating eosinophils are increased in ADe patients compared with ADi patients.