Distribution of estrogen receptor alpha in the dominant follicles and corpus luteum at the three stages of estrous cycle in Japanese Black cows
Distribution of estrogen receptor alpha in the dominant follicles and corpus luteum at the three stages of estrous cycle in Japanese Black cows
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DOI：
10.1292/jvms.66.1183
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发表时间：
2004-10-01
影响因子：
1.2
通讯作者：
Hamana, K
中科院分区：
文献类型：
--
作者：
Amrozi;Kamimura, S;Hamana, K
应用免疫组织化学方法研究了日本黑牛发情周期3个阶段优势卵泡（DF）和黄体（CL）中雌激素受体α（ER α）的分布。每天两次使用超声检查观察卵巢动力学，直到在第7天（第一组，n=3）、第10天（第二组，n=3）和第18天（第三组，n=3）（第0天=发情）进行卵巢切除术。使用配备有数码相机的光学显微镜测定细胞中以免疫组织学染色强度表示的ER α表达。与壁颗粒细胞（mGC）、窦颗粒细胞（aGC）和外膜细胞（TE）相比，DF内膜（TI）中的表达有增高的趋势。第三组ER α表达在mGC中低于第一组，在TE中也低于第二组（P
Distribution of estrogen receptor alpha (ERalpha) in the dominant follicle (DF) and corpus luteum (CL) at the three stages of estrous cycle in Japanese Black cows was evaluated by means of immunohistochemistry. Ovarian dynamics were observed twice daily using ultrasonography until the ovariectomy performed on Day 7 (First group, n=3), Day 10 (Second group, n=3) and Day 18 (Third group, n=3) (Day 0=estrus). Expression of ERalpha represented by immunohistological staining intensity in cells was determined using a light microscope equipped with a digital camera. A tendency toward higher expression were observed in theca interna (TI) of DF when compared with those in mural granulosa cells (mGC), antral granulosa cells (aGC) and theca externa (TE). ERalpha expression in the Third group was lower than that in the First Group in mGC, and it was also lower than that in the second group in TE (P