Antioxidant, antibacterial and anti-cancer activities of β-and γ-CDs/curcumin loaded in chitosan nanoparticles
Antioxidant, antibacterial and anti-cancer activities of β-and γ-CDs/curcumin loaded in chitosan nanoparticles
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DOI：
10.1016/j.ijbiomac.2020.01.206
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发表时间：
2020-03-15
影响因子：
8.2
通讯作者：
Malakzadeh, Shokufeh
中科院分区：
文献类型：
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作者：
Alizadeh, Nina;Malakzadeh, Shokufeh
姜黄素是一种天然多酚类化合物，由于其具有保护细胞免受氧化损伤的能力，已被广泛用作抗癌和抗氧化剂。在本研究中，我们制备了高度可溶性的CUR-beta-环糊精（3-CD）和γ-环糊精（γ-CD）空心球。紫外-可见分光光度法证实了环糊精的疏水空腔包裹了CUR的芳香环形成包合物。然后将CUR-CD包封到带正电荷的可生物降解壳聚糖（CUR-CD-CS）纳米颗粒中。采用UV-Vis、FTIR、（HNMR）-H-1、XRD、SEM等手段对CUR-CD-CS进行了表征。通过测量抑菌圈标度和MIC值来评价抗菌功效，MIC值记录为32 μ g/mL和64 pg/mL，用于两种革兰氏阳性S.金黄色葡萄球菌和革兰氏阴性E.分别称为细菌。我们使用MU测定法测试了这些CUR-CD-CS纳米颗粒在A549细胞系中的功效，并研究了其细胞摄取机制。我们的结果表明，CUR-CD-CS纳米粒在所有测试的制剂中显示出上级的体外释放性能和在A549细胞系中的更高的细胞毒性。研究了载姜黄素的CDs-CS的抗氧化活性和体外释放行为。采用DPPH法测定了载姜黄素的CDs-CS/CUR的IC 50值。(C)2020 Elsevier B. V.保留所有权利。
Curcumin, as a naturally occurring polyphenol, has been extensively used as anticancer and antioxidant agent due to its ability to protect cells from oxidative damage. In the present study, we have prepared highly soluble CUR-beta-cydodextrin (3-CD) and gamma-cyclodextrin (gamma-CD) hollow spheres. UV-Vis method was employed to approve the successful formation of the inclusion complex where the aromatic ring of CUR has been encapsulated by the hydrophobic cavity of CDs. CUR-CDs were then encapsulated into positively charged biodegradable chitosan (CUR-CDs-CS) nanopartides. The CUR-CD-CS were characterized by UV-Vis, FTIR, (HNMR)-H-1, XRD, SEM analysis. Antibacterial efficacy was evaluated by measuring the zone of inhibition scale and MIC value which was recorded as 32 mu g/mL and 64 pg/mL for both Gram positive S. aureus and Gram negative E. call bacteria respectively. We tested the efficacy of these CUR-CD-CS nanopartides in A549 cell lines using MU assay and investigated its cellular uptake mechanism. Our results demonstrated that CUR-CD-CS nanoparticles showed superior in vitro release performance and higher cytotoxicity in A549 cell line among all tested formulations. Antioxidant activity and release behavior of the curcumin-loaded CDs-CS was investigated The IC50 value for CUR/CD-CS was estimated based on their inhibition percent-concentration curves using DPPH assay. (C) 2020 Elsevier B.V. All rights reserved.