Quick and simple sample treatment for multiresidue analysis of bisphenols, bisphenol diglycidyl ethers and their derivatives in canned food prior to liquid chromatography and fluorescence detection
Quick and simple sample treatment for multiresidue analysis of bisphenols, bisphenol diglycidyl ethers and their derivatives in canned food prior to liquid chromatography and fluorescence detection
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DOI：
10.1016/j.chroma.2014.02.008
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发表时间：
2014-04-04
影响因子：
4.1
通讯作者：
Rubio, S.
中科院分区：
文献类型：
--
作者：
Alabi, A.;Caballero-Casero, N.;Rubio, S.
本文报道了一种用于罐头食品中12种双酚[双酚a （BPA）、双酚B （BPB）、双酚F （BPF）、双酚E (BPE)]、双酚二缩水甘油酯醚[双酚F二缩水甘油酯醚（BFDGE）、双酚a二缩水甘油酯醚（BADGE）]及其衍生物[BADGE中心点2H(2)O、BADGE中心点H2O、BADGE中心点HCl、BADGE中心点HCl、BADGE中心点2HCl和BFDGE中心点2HCl]的多残留测定方法。该方法以600 μ L由十四醇反聚集体组成的超分子溶剂对200 mg食品样品中的目标污染物进行微萃取，然后采用外标液相色谱/荧光检测对萃取物进行分析。所有目标化合物，包括BFDGE和BFDGE中心点2HCl的邻位、对位和对位异构体，均以基线分离（分辨率>= 1.52）实现色谱分离。提取液无需浓度，微萃取时间约30 min，可同时处理多个样品。方法验证根据欧盟委员会决议2002/657/EC的建议进行。不同分析物的定量限在0.9 ~ 3.5 μ g kg（-1）之间。重复性和再现性（以相对标准偏差表示）分别为1.8 ~ 6.8%和4.4 ~ 8.1%。该方法应用于蔬菜、豆类、水果、鱼类和海鲜、肉制品和谷物等不同食品类别的目标化合物分析。样品加样回收率在80 ~ 110%范围内。罐头食品中未检出BPF和BPE。其余双酚类、二缩水甘油酯及其衍生物的浓度在7.1-959 μ g kg（-1）之间。对BFDGE和BFDGE中心点2HCl在食品中的同分异构体分布的研究表明，它们优先作为一种同分异构体形式存在，值得进一步研究。这种多残留方法的分析和操作特性使其适合于用于评估人体暴露于饮食中的双酚、二缩水甘油醚和衍生物的监测程序。(C) 2014 Elsevier B.V.版权所有
We report herein a multiresidue method for canned food determination of 12 bisphenols [bisphenol A (BPA), bisphenol B (BPB), bisphenol F (BPF), bisphenol E (BPE)], bisphenol diglycidyl ethers [bisphenol F diglycidyl ether (BFDGE), bisphenol A diglycidyl ether (BADGE)] and their derivatives [BADGE center dot 2H(2)O, BADGE center dot H2O, BADGE center dot HCl center dot H2O, BADGE center dot HCl, BADGE center dot 2HCl and BFDGE center dot 2HCl]. The method was based on the microextraction of the target contaminants in 200 mg food sample with 600 mu L of a supramolecular solvent made up of inverse aggregates of tetradecanol, followed by analysis of the extract by liquid chromatography/fluorescence detection using external calibration. Chromatographic separation of all target compounds, including the ortho-ortho, ortho-para and para-para isomers of BFDGE and BFDGE center dot 2HCl, was achieved with baseline separation (Resolution >= 1.52). No concentration of the extracts was required, the microextraction took about 30 min and several samples could be simultaneous treated. Method validation was carried out according to the recommendations of the European Commission Decision 2002/657/EC. Quantitation limits for the different analytes ranged between 0.9 and 3.5 mu g kg(-1). Repeatability and reproducibility, expressed as relative standard deviation, were in the ranges 1.8-6.8% and 4.4-8.1%. The method was applied to the analysis of the target compounds in different food categories including vegetables, legumes, fruits, fish and seafood, meat product and grain. Recoveries in samples were within the range 80-110%. Only BPF and BPE were undetected in the canned food analyzed. The concentration found for the rest of bisphenols, diglycidyl ethers and derivatives was in the range 7.1-959 mu g kg(-1). The study of the isomeric distribution of BFDGE and BFDGE center dot 2HCl in food showed that they are preferentially present as one of the isomeric forms, that highlighting for further studies. The analytical and operational characteristics of this multiresidue method make it suitable for monitoring programs intended for the assessment of human exposure to bisphenols, diglycidyl ethers and derivatives from diet. (C) 2014 Elsevier B.V. All rights reserved.